Method for rapidly establishing medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants

A medium-sized pennisetum, tissue culture technology, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problems of restricting research development, tissue culture and reproduction, time-consuming and laborious, slow callus and differentiation process, etc. , to achieve the effect of simple operation and easy completion

Inactive Publication Date: 2015-06-24
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the rapid propagation of Pennisetum tissue can be carried out indoors, the method of tissue culture and propagation is time-consuming and laborious, and cannot fundamentally solve the problem of large-scale propagation of medium-sized Pennisetum. The problem is to improve its seed firmness and germination rate. the key to
However, the molecular mechanism of its seed setting and low germination rate has not been studied clearly at present. The important problem is the basic conditions for its molecular mechanism research---the tissue culture regeneration system of Pennisetum with seeds as explants has not yet been established. Established, to a large extent, limited the development of relevant research, and the callus and differentiation process is relatively slow in the traditional petri dish sealed culture mode, and there is currently a lack of a medium-sized Pennisetum tissue that uses seeds as explants to quickly establish Methods for cultivating regenerative systems

Method used

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  • Method for rapidly establishing medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants
  • Method for rapidly establishing medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants
  • Method for rapidly establishing medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants

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Experimental program
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Effect test

Embodiment 1

[0027] A kind of medium-sized Pennisetum tissue culture regeneration method using seeds as explants of the present invention is as follows:

[0028] (1) Selection, preparation and disinfection of raw materials

[0029] Select mature and plump seeds and wash them several times with tap water, then place them in the ultra-clean workbench, disinfect them with 75% alcohol for 3 minutes, sterilize with 5% sodium hypochlorite (NaClO) for 10 minutes, rinse them with sterile water for 3 to 5 times, and place them on the ultra-clean workbench Soak in sterile deionized water for 8 hours to absorb swelling.

[0030] (2) Inoculation and culture of callus

[0031] The seeds in step (1) were treated with MS medium + 1.0% sucrose + 0.5% agar + 2.0mg / L 2,4-D + 1.0mg / L KT, and the pH value was adjusted to 6.0 as medium-sized Pennisetum wild The induction medium of the callus of seedling, the temperature is 24 ℃. Then the sterilized seeds are inoculated on the above-mentioned medium, based o...

Embodiment 2

[0040] A kind of medium-sized Pennisetum tissue culture regeneration method using seeds as explants of the present invention is as follows:

[0041] (1) Selection, preparation and disinfection of raw materials

[0042] Select mature and plump seeds and wash them several times with tap water, then place them in the ultra-clean workbench, disinfect them with 75% alcohol for 3 minutes, sterilize with 5% sodium hypochlorite (NaClO) for 10 minutes, rinse them with sterile water for 3 to 5 times, and place them on the ultra-clean workbench Soak in sterile deionized water for 8 hours to absorb swelling.

[0043] (2) Inoculation and culture of callus

[0044] The seeds in step (1) were treated with MS medium+2.0% sucrose+1.0% agar+5.0mg / L 2,4-D+1.5mg / L KT, and the pH value was adjusted to 5 as medium-sized Pennisetum wild The induction medium of the callus of seedling, the temperature is 26 ℃. Then the sterilized seeds are inoculated on the above-mentioned medium, and based on the ...

Embodiment 3

[0053] A kind of medium-sized Pennisetum tissue culture regeneration method using seeds as explants of the present invention is as follows:

[0054] (1) Selection, preparation and disinfection of raw materials

[0055] Select mature and plump seeds and wash them several times with tap water, then place them in the ultra-clean workbench, disinfect them with 75% alcohol for 3 minutes, sterilize with 5% sodium hypochlorite (NaClO) for 10 minutes, rinse them with sterile water for 3 to 5 times, and place them on the ultra-clean workbench Soak in sterile deionized water for 12 hours to absorb swelling.

[0056] (2) Inoculation and culture of callus

[0057] The seeds in step (1) were treated with MS medium+3.0% sucrose+0.7% agar+3.0mg / L 2,4-D+2.0mg / L KT, and the pH value was adjusted to 5.8 as medium-sized pennisetum wild The induction medium of the callus of seedling, the temperature is 24 ℃. Then the sterilized seeds were inoculated on the above-mentioned culture medium. Based...

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Abstract

The invention provides a method for rapidly establishing a medium-sized Chinese pennisetum herb tissue culture regeneration system by taking seeds as explants. The method mainly comprises the following steps: performing selection, disinfection and soaking on a medium-sized Chinese pennisetum herb raw material; performing inoculation, culture and differentiation on callus tissues; performing rooting culture on tissue culture seedlings; and performing acclimatization and transplanting. By adopting the method provided by the invention, an indoor rapid tissue culture regeneration system of medium-sized Chinese pennisetum herb wild species can be rapidly established, and the indoor rapid tissue culture regeneration system is an important foundation for further researching a molecular mechanism with low seed setting and germination rate; and moreover, the method is simple to operate and easy to complete.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for quickly establishing a medium-sized pennisetum tissue culture regeneration system using seeds as explants. Background technique [0002] Medium Pennisetum ( Pennisetum longissimum var.intermedium) is a perennial wild herbaceous plant of Pennisetum genus Poaceae, which is distributed in Yunnan, Sichuan, Guizhou, Hunan and other places. The medium-sized pennisetum has thicker stems, well-developed root system, wide adaptability, strong regeneration ability, high grass yield, relatively high nutritional content, and it has strong disease resistance, drought resistance, humidity resistance and salt-alkali tolerance. It is an important species for the promotion of high-quality forage and for the study of important mechanisms such as salt tolerance, drought resistance, and high yield of gramineous plants. However, due to its low seed setting rate and germination rate in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王春梅田福平张怀山王晓力张茜朱新强杨晓伍国强袁慧君杨红善胡宇周学辉
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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