Applications of expression vector carrying light-sensing ion channel protein encoding gene in preparation of respiratory rhythm regulating and controlling drug

An ion channel protein and respiratory rhythm technology, which is applied in the application field of an expression vector carrying a gene encoding a light-sensitive ion channel protein in the preparation of a drug for regulating respiratory rhythm, and can solve the problems of insufficient breathing and ventilation, diaphragmatic muscle fatigue, and unsatisfactory improvement effect. , to achieve the effect of slowing down the breathing rate, breathing evenly, and avoiding apnea

Inactive Publication Date: 2015-06-24
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In addition, continuous stimulation of the phrenic nerve can lead to fatigue of the diaphragm. After a long period of time, the respiratory volume will decrease, which will still cause hypoventilation, which is not ideal for the improvement of the previous symptoms.

Method used

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  • Applications of expression vector carrying light-sensing ion channel protein encoding gene in preparation of respiratory rhythm regulating and controlling drug
  • Applications of expression vector carrying light-sensing ion channel protein encoding gene in preparation of respiratory rhythm regulating and controlling drug
  • Applications of expression vector carrying light-sensing ion channel protein encoding gene in preparation of respiratory rhythm regulating and controlling drug

Examples

Experimental program
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Effect test

Embodiment 1

[0053] The application of the expression vector carrying the gene encoding ChR2 in the preparation of drugs for regulating respiratory rhythm specifically includes the following steps: firstly, mixing the lentiviral vector carrying the gene encoding ChR2 with deionized water to prepare a mixed solution is the drug for regulating respiratory rhythm. C57 / BL6 mice were anesthetized with anesthesia and fixed on a stereotaxic apparatus. The scalp was cut, the position of the anterior nucleus of the thalamus was determined according to the brain stereotaxic atlas, and a hole was drilled. Electrodes are inserted into the diaphragm to monitor breathing changes. Inject the drug for regulating respiratory rhythm in the anterior nucleus of the mouse thalamus (lentivirus titer is 10 10 ). Different concentrations of isoflurane gas were anesthetized throughout the experiment to maintain the level of anesthesia under light anesthesia. The lentiviral vector carrying the ChR2 gene will exp...

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Abstract

Embodiments of the invention provide applications of an expression vector carrying a light-sensing ion channel protein encoding gene in preparation of a respiratory rhythm regulating and controlling drug. According to the present invention, the respiratory rhythm regulating and controlling drug provides effects for gamma-GABAergic nerve fibers in thalamus anterior nucleus so as to effectively slow down the respiration rate and make breath be uniform; and tidal respiration, sighing respiration and other diseases related to unstable respiratory rhythm can be effectively improved, the body can be controlled to stably intake a proper amount of oxygen so as not to intake excessive oxygen and too little carbon dioxide, the always exciting of the respiratory center is ensured, the early prevention is provided so as to avoid the apnea phenomenon, and safety and reliability are provided.

Description

technical field [0001] The invention relates to the application of an expression vector carrying a gene encoding a light-sensitive ion channel protein in the preparation of drugs for regulating respiratory rhythm. Background technique [0002] Breathing is a kind of rhythmic movement. At present, the study believes that the breathing rhythm is produced by the respiratory nerve cell group in the respiratory center, participates in the regulation and improvement of the breathing rhythm, and realizes the normal breathing movement of the body together. Due to organ damage such as heart and lung failure, tracheal blockage, pneumothorax, high cerebral pressure and reduced sensitivity to carbon dioxide, etc., the respiratory rhythm is damaged, and shallow and fast breathing, deep and slow breathing, apnea, tidal breathing, Sighing breathing, etc., can lead to serious consequences such as respiratory acidosis, alkalosis, respiratory arrest and death. In traditional treatment method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K47/46A61P11/16
Inventor 张奕王立平李娟蔚鹏飞任锋孙鹏鲁艺刘磊
Owner SHENZHEN INST OF ADVANCED TECH
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