Respiratory rhythm regulation medicine, and preparation method and application thereof

A respiratory rhythm and drug technology, applied in the direction of drug combination, medical formula, respiratory system diseases, etc., can solve problems such as unsatisfactory improvement effect, diaphragm muscle fatigue, and insufficient ventilation

Inactive Publication Date: 2015-06-24
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, continuous stimulation of the phrenic nerve can lead to fatigue of the diaphragm. After a long period of time, the respiratory volume will decrease, which will still cause hypoventilation, which is not ideal for the improvement of the previous symptoms.

Method used

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  • Respiratory rhythm regulation medicine, and preparation method and application thereof
  • Respiratory rhythm regulation medicine, and preparation method and application thereof
  • Respiratory rhythm regulation medicine, and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0034] The second aspect of the embodiment of the present invention provides a method for preparing a drug for regulating respiratory rhythm, comprising the following steps:

[0035] A drug capable of regulating the excitability of γ-aminobutyric acid neurons is mixed with a drug carrier to prepare a drug for regulating respiratory rhythm, and the substance capable of regulating the excitability of γ-aminobutyric acid neurons is γ-aminobutyric acid , γ-aminobutyric acid receptor agonist or an expression vector carrying a gene encoding a light-sensitive ion channel protein, the pharmaceutical carrier is an aqueous solvent, a non-aqueous solvent, a suspension or an emulsion.

[0036] The γ-aminobutyric acid receptor agonist is muscimol.

[0037]The gene encoding light-sensitive ion channel protein is the gene encoding ChR2 or the gene encoding the same function as ChR2.

[0038] The expression vector is a plasmid, a viral vector or a non-viral vector. The viral vector is a len...

Embodiment 1

[0053] The application of gamma-aminobutyric acid receptor agonists in the preparation of drugs for regulating respiratory rhythm specifically comprises the following steps:

[0054] Mixing muscimol and deionized water to prepare a mixed solution is the drug for regulating respiratory rhythm, wherein the concentration of muscimol is 0.3 μg / μl. C57 / BL6 mice were anesthetized with anesthesia and fixed on a stereotaxic apparatus. The scalp was cut, the position of the anterior nucleus of the thalamus was determined according to the brain stereotaxic atlas, and a hole was drilled. Electrodes are inserted into the diaphragm to monitor breathing changes. The drug for regulating respiratory rhythm was injected into the anterior nucleus of the mouse thalamus. Different concentrations of isoflurane gas were anesthetized throughout the experiment to maintain the level of anesthesia under light anesthesia.

[0055] figure 1 It is a schematic diagram of the respiratory rate measured a...

Embodiment 2

[0058] The application of the expression vector carrying the gene encoding ChR2 in the preparation of drugs for regulating respiratory rhythm specifically includes the following steps: firstly, mixing the lentiviral vector carrying the gene encoding ChR2 with deionized water to prepare a mixed solution is the drug for regulating respiratory rhythm. C57 / BL6 mice were anesthetized with anesthesia and fixed on a stereotaxic apparatus. The scalp was cut, the position of the anterior nucleus of the thalamus was determined according to the brain stereotaxic atlas, and a hole was drilled. Electrodes are inserted into the diaphragm to monitor breathing changes. Inject the drug for regulating respiratory rhythm in the anterior nucleus of the mouse thalamus (lentivirus titer is 10 10 ). Different concentrations of isoflurane gas were anesthetized throughout the experiment to maintain the level of anesthesia under light anesthesia. The lentiviral vector carrying the ChR2 gene will exp...

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Abstract

The embodiment of the invention provides a respiratory rhythm regulation medicine. The respiratory rhythm regulation medicine comprises a substance having a gamma-aminobutyric acid ergic neuron excitement degree regulating ability, and a medicine carrier, the substance having a gamma-aminobutyric acid ergic neuron excitement degree regulating ability is gamma-aminobutyric acid, a gamma-aminobutyric acid receptor stimulant or an expression vector carrying light-sensitive ion channel protein encoding gene, and the medicinal carrier is an aqueous solvent, a non-aqueous solvent, a suspension or an emulsion. The respiratory rhythm regulation medicine can effectively alleviate the breathing frequency to make the breath uniform, can effectively improve tidal breathing, sighing breath and other unstable respiratory rhythm related diseases, can control body to stably intake a proper amount of oxygen to avoid too much oxygen and too less carbon dioxide, guarantees excitement keeping of the respiratory center, prevents an apnoea phenomenon, and is safe and reliable.

Description

technical field [0001] The invention relates to a drug for regulating breathing rhythm, its preparation method and application. Background technique [0002] Breathing is a kind of rhythmic movement. At present, the study believes that the breathing rhythm is produced by the respiratory nerve cell group in the respiratory center, participates in the regulation and improvement of the breathing rhythm, and realizes the normal breathing movement of the body together. Due to organ damage such as heart and lung failure, tracheal blockage, pneumothorax, high cerebral pressure and reduced sensitivity to carbon dioxide, etc., the respiratory rhythm is damaged, and shallow and fast breathing, deep and slow breathing, apnea, tidal breathing, Sighing breathing, etc., can lead to serious consequences such as respiratory acidosis, alkalosis, respiratory arrest and death. In traditional treatment methods, the main targets are the medullary respiratory center, cerebral cortex, aortic body...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/42A61K31/197A61P11/00
Inventor 张奕王立平周意蔚鹏飞任锋孙鹏李娟鲁艺刘磊
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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