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Serratia marcescens strain resistant to xanthomonas axonopodis pv. citri

A technology for the citrus canker fungus, Serratia marcescens, in the direction of bacteria, microbe-based methods, chemicals for biocontrol, etc.

Inactive Publication Date: 2015-06-24
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rossetti (1977), Civerolo (1981), Schoulties (1987), Civerolo (1991) and Goto (1992) all expounded the views on the prevention and treatment of ulcer disease in their studies, but their studies were all restricted by specific regions. Restrictions may be outdated in today's era of deep communication

Method used

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  • Serratia marcescens strain resistant to xanthomonas axonopodis pv. citri
  • Serratia marcescens strain resistant to xanthomonas axonopodis pv. citri
  • Serratia marcescens strain resistant to xanthomonas axonopodis pv. citri

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 Preparation of antagonistic bacterial strain T861 of the present invention

[0030] Collect soil samples from Longshan County, Xiangxi Autonomous Prefecture. Take 10g of each soil sample, add them to a triangular flask filled with 90ml sterile water (with glass beads), shake for 1 hour, filter to remove sediment, and use sterile water to obtain the bacterial suspension. Carry out serial dilution, successively dilute to 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 . Bacteria were separated by mixing method, and fungi and actinomycetes were separated by smear method. Cultivate at 28°C for 48 hours, select colonies with different shapes, sizes, and colors, purify them, and store them in slant medium.

[0031] The citrus canker sore bacteria is mixed with the melted culture medium and poured into a sterilized petri dish, a hole is punched on the solidified gel plate, and the isolated strains are made into a certain concentration of bacterial suspension , use a pipe...

Embodiment 2

[0032] Example 2 Indoor inhibitory effect of bacterial strain T861 of the present invention on canker sore citrus

[0033] Mix the bacteria solution made by X. citrus canker sore with the melted culture medium, pour it into a sterilized petri dish, make a hole on the solidified gel plate, and make the isolated T861 strains into suspensions of certain concentrations. solution, use a pipette gun to take 100ul of the bacterial solution and add it to the round hole of the corresponding plate, and set up 3 parallel holes. Place the marked Petri dish in a 30°C incubator, and observe the formation of the inhibition zone every 24 hours. The average value of the diameter of the inhibition zone formed by strain T861 is about 55mm, and the edge of the inhibition zone is slightly raised, see figure 2 .

Embodiment 3

[0034] Example 3 Bacterial strain T861 of the present invention is used for preventing and treating canker sores of citrus on living citrus

[0035] The bacterial strain T861 of the present invention was picked and inoculated into 25ml of liquid LB liquid medium, cultured at 37°C and 180r / min for 24h to obtain a fermentation broth. Select 3 citrus trees with similar growth conditions, select four fully expanded new leaves with similar growth and development levels on each tree, and puncture the upper epidermis of the leaves with a sterilized inoculation needle (20 holes per leaf). Spray X. citrus bacteria on the upper and lower surfaces of the leaves by spraying. After 1 day, spray the fermented liquid of the bacterial strain T861 of the present invention on three new leaves of citrus treated by X. citrus. , do not spray the fermented liquid of bacterial strain of the present invention but spray clear water on the control blade, observe the disease situation after spraying bac...

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PUM

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Abstract

A serratia marcescens strain T861 resistant to xanthomonas axonopodis pv. citri is preserved in CCTCC (china center for type culture collection) on 10, April, 2015, with the number of CCTCC M2015222. Fermentation liquor of the serratia marcescens strain T861 can suppress Xanthomonas axonopodis pv. citri; measurement by means of needle piercing shows that aseptic fermentation liquor of the serratia marcescens strain T861 has an in-vitro suppression rate for Xanthomonas axonopodis pv. citri being above 86.7%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a Serratia marcescens strain resistant to canker sores of citrus, in particular to a Serratia marcescens T861 strain, and to the prevention and treatment of canker citrus by the strain at the same time Applications. Background technique [0002] Citrus canker is a quarantine disease caused by Xanthomonas axonopodis pv. Citri, which seriously affects the quality and yield of citrus. It has been reported in most parts of the world where citrus crops grow. Citrus canker is a bacterial disease, which has the characteristics of fast infection, wide transmission route, high degree of damage, and stubborn disease. Although countries around the world have formulated strict regulations and adopted various measures to block the introduction of canker disease, the adjustment of agricultural and forestry industry structure and the development of economic trade have accelerated the pace of infecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/43
CPCA01N63/00A01N63/10C12N1/205C12R2001/43
Inventor 唐前君罗葵戴良英周志成肖启明刘双清李魏李迅
Owner HUNAN AGRICULTURAL UNIV
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