Isoflavone compound with anti-virus activity as well as preparation method and application thereof

A technology of isoflavones and compounds, applied in the field of isoflavones, to achieve the effect of good activity, simple structure and good anti-rotavirus activity

Active Publication Date: 2015-07-08
CHINA TOBACCO YUNNAN IND
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention isolates an isoflavone compound with anti-rotavirus activity from tobacco, which has not been reported so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Isoflavone compound with anti-virus activity as well as preparation method and application thereof
  • Isoflavone compound with anti-virus activity as well as preparation method and application thereof
  • Isoflavone compound with anti-virus activity as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of isoflavone compound C 18 h 16 o 6 , including extracting extract, silica gel column chromatography, and high-pressure liquid chromatography separation, specifically adopting the following steps:

[0032] 1. Extraction of extract: take the whole plant of the tobacco sample, the tobacco can be crushed or cut into small sections, and the mass percentage concentration is 70% to 95% methanol or ethanol, or the mass percentage concentration is 60% to 95% acetone. Any kind of extraction solvent, each extraction soaks tobacco powder or small pieces in the extraction solvent for 54h, extracts 3 times, the weight ratio of the amount of each extraction solvent to tobacco=2.5:1, merges the extracts, filters, and concentrates the filtrate into extract;

[0033] 2. Silica gel column chromatography: dissolve the extract with 2.5 times the weight of pure methanol, pure ethanol or pure acetone, mix the sample with 80-100 mesh silica gel 1.2 times the weight of the extr...

Embodiment 2

[0038] The tobacco samples were from Yuxi, Yunnan, and the variety was Yuxi K326. Sampling 2.0 kg of tobacco and pulverizing it with 95% methanol by mass, extracting it 5 times, each time for 24 hours, the weight ratio of the amount of 95% methanol to tobacco = 2:1, the extract Combined, filtered, and the filtrate was concentrated under reduced pressure to form an extract to obtain 100 g of extract.

[0039] After the extractant is dissolved in pure methanol whose weight is 2.0 times of its amount, mix the sample with 120g of 100 mesh thick silica gel, pack 0.6kg of 160 mesh silica gel column for silica gel column chromatography, and use a volume ratio of 1:0, 20: 1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone mixed solvent gradient elution, when each ratio of solvent elution, elution until there is no component elution Up to the bottom, then change the solvent of the next ratio, TLC monitoring and merging the same parts to obtain 8 parts, wherein the chloroform-acetone e...

Embodiment 3

[0041] The tobacco sample comes from Dali, Yunnan, and the variety is Yunyan 200. The tobacco sample is 3.5kg and cut into small pieces, extracted 4 times with 95% ethanol, each time for 48 hours, the weight ratio of the amount of 95% ethanol to tobacco = 3 : 1, the extracts are combined, filtered, and the filtrate is concentrated under reduced pressure into an extractum to obtain extractum 145g.

[0042] The extract is dissolved in pure ethanol that is 1.5 times its weight, and then mixed with 150g of 80-mesh silica gel, and 1.0kg of 200-mesh silica gel is packed into a column for silica gel column chromatography. The volume ratio is 1:0, 20:1 , 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, each ratio of solvent elution, elution until no component is washed down, Then change the solvent of the next ratio, TLC monitors and merges the same parts to obtain 8 parts, wherein the chloroform-acetone eluting part with a volume ratio of 8:2 is separated by Agilent ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
degree of unsaturationaaaaaaaaaa
Login to view more

Abstract

The invention discloses an isoflavone compound shown in a formula (I) in the specification as well as a preparation method and application thereof. The preparation method comprises the following steps: by taking a total tobacco plant as a raw material and taking a mixed solvent of methyl alcohol-water, ethyl alcohol-water or acetone-water as an extracting solvent, performing extraction, combining extracting solutions, filtering, and performing reduced pressure concentration on a filtrate to prepare an extract; performing silica-gel column chromatography on the extract by using silica-gel dry-process column packing; performing gradient elution by using a mixed solvent of trichloromethane-acetone; and further performing high-pressure liquid chromatographic separation and purification on an 8:2 part of an eluent to prepare the required isoflavone compound. Activity tests show that the compound disclosed by the invention has a very good inhibition effect on rotavirus; and the compound disclosed by the invention is simple in structure and good in compound activity, and can be used as an anti-rotavirus lead compound.

Description

technical field [0001] The invention belongs to the technical field of tobacco chemistry, in particular to an isoflavone compound extracted from tobacco for the first time. At the same time, the invention also relates to the preparation method and application of the compound. Background technique [0002] Tobacco is the plant with the most complex chemical composition in the world, and its secondary metabolites are very rich. After decades of research, more than 3,000 monomeric chemical substances have been identified from tobacco, and many components have not yet been identified. come out. In addition to being mainly used for smoking cigarettes, tobacco can also extract a variety of valuable chemical components from it, and discover leading compounds that are valuable for development and utilization. [0003] Isoflavones are compounds ubiquitous in a class of natural plants, and there are also reports of such compounds in tobacco. Isoflavones have a wide range of pharmaco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36C07D311/40A61P31/14
CPCC07D311/36C07D311/40
Inventor 刘春波申钦鹏杨光宇张凤梅何沛张涛司晓喜苏钟璧刘志华朱瑞芝王昆淼缪明明
Owner CHINA TOBACCO YUNNAN IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap