Method for preparing scanning electron microscope sample of silage corn leaf

A technology of silage corn and scanning electron microscope, which is applied in the preparation of test samples and other directions, can solve the problems of unclear images and incomplete fiber structure of plant leaves, and achieves the advantages of strong image three-dimensional effect, clear sample structure and simple and convenient preparation method. Effect

Inactive Publication Date: 2015-07-15
GANSU AGRI UNIV
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Problems solved by technology

[0004] At present, the research technology on the microstructure of plant leaves after livestock rumen degradation is still immature. The samples obtained by the conventional method of preparing scanning electron microscope observation samples have incomplete fiber structure of plant leaves and incomplete images when observed under scanning electron microscope. clear question

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  • Method for preparing scanning electron microscope sample of silage corn leaf
  • Method for preparing scanning electron microscope sample of silage corn leaf
  • Method for preparing scanning electron microscope sample of silage corn leaf

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Embodiment Construction

[0032] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0033] The experimental methods that do not indicate the specific conditions in the following examples are usually in accordance with the conventional conditions or the conditions suggested by the manufacturer.

[0034] making process:

[0035] Step 1. Prepare fixative

[0036] Take 25v% glutaraldehyde solution, 0.2M phosphate buffer and redistilled water, prepare in proportion to obtain 2.5v% glutaraldehyde fixative.

[0037] Step 2. Cleaning:

[0038] The silage corn leaves degraded by the rumen of domestic animals (sheep were used in this example) were gently dialed one by one with tweezers into the penicillin vial filled with 0.1mol / L pH 7.2 phosphate buffer for rinsing, and the buffer was repeatedly changed until The blade surface is clean, if it is not cleaned, it will affect the follow-up photographing effect. Generally, it is r...

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Abstract

The invention discloses a method for preparing a scanning electron microscope sample of a silage corn leaf. According to the method, the prepared scanning electron microscope sample is made to be complete in structure through the processes of washing, dehydration, de-ethanol, freezing, drying, gold plating and the like, when scanning electron microscope observation is carried out, the surface structure of the leaf is clear and complete, the stereoscopic impression of an image is strong, observation and research for the ultra microstructure of the silage corn leaf after ruminal degradation of livestock are convenient to carry out, and the method has important significance for visually knowing and evaluating the degradation of rumen microbe of livestock to plant leaf fiber structures and improving the digestibility of straw fodder.

Description

technical field [0001] The invention relates to a method for preparing scanning electron microscope samples, in particular to a method for preparing livestock rumen-degraded silage corn leaf scanning electron microscope samples, and belongs to the technical field of microscopic observation samples. Background technique [0002] As a non-competitive resource, crop straw is a non-competitive resource. The annual output of crop straw in my country is nearly 600 million tons to 700 million tons. Most of the straw resources are not effectively utilized. Due to the rough stems and high crude fiber content of straw, it is difficult for animals to digest utilization, resulting in huge waste and environmental pollution. Through the analysis of straw fiber structure technology, it will bring huge social and economic benefits. [0003] Silage corn does not refer to corn varieties, but in view of the purpose, fresh corn is stored in the silo (for silage) and fermented for a period of ti...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 焦婷梁建勇吴建平赵生国
Owner GANSU AGRI UNIV
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