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Gene typing detection kit for human 13,18 and 21 chromosome 20 STR locus

A technology of D13S1817 and gene loci, which is applied in the field of genotyping detection kits, can solve problems such as application troubles, and achieve the effects of good typing maps, improved work efficiency, and cost savings

Active Publication Date: 2015-08-05
上海春夏正像生物科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

However, this characteristic of the STR locus has caused problems in its application in the identification of kinship

Method used

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  • Gene typing detection kit for human 13,18 and 21 chromosome 20 STR locus
  • Gene typing detection kit for human 13,18 and 21 chromosome 20 STR locus
  • Gene typing detection kit for human 13,18 and 21 chromosome 20 STR locus

Examples

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Effect test

Embodiment

[0037] Example 1 : Human blood sample genome dna preparation

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Abstract

The invention belongs to the technical filed of human nucleic acid in-vitro detection, and more specifically relates to a gene typing detection kit for human 13,18 and 21 chromosome 20 STR locus. According to the invention, a composite PCR amplification system for gene typing on 20 STR locus distributed on human 13, 18 and 21 chromosome is designed, and wherein a PCR amplification primer group is SEQ ID No.1-SEQ ID No. 40. The gene typing detection kit comprises a primer combination container and a PCR reaction mother liquor container; and the primer combination container comprises primer SEQ ID No.1-SEQ ID No. 40 composition storage liquid. In the invention, single tube amplification on 20 STR locus is carried out, STR locus in each fluorescence channel and different fluorescence channels have equal amplification, a typing graph is good; cost, manpower and time can be obviously saved, and work efficiency is increased.

Description

technical field [0001] The invention belongs to the technical field of in vitro detection of human nucleic acid, in particular to the detection of the genotype of the highly polymorphic STR loci in human genome DNA, and in particular to a method for detecting human No. 13, 18 and 21 by multiple polymerase chain reaction Kit for genotyping detection of 20 STR loci on chromosomes. Background technique [0002] Short tandem repeats (Short tandem repeats, STR) are a type of DNA tandem repeats that widely exist in the human genome. The core repeat unit is usually 2-6 bases, and it is the most polymorphic genetic sequence in the human genome. Markers, the polymorphism of which is mainly derived from the difference in the number of repeats of the core repeat unit among individuals, and the repeat number of the core repeat unit follows the Mendelian law of inheritance in the genetic process. Because alleles of the same STR locus (STR locus alleles are usually named according to the...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156C12Q2600/16
Inventor 赵书民周巍龚虎涛
Owner 上海春夏正像生物科技有限公司
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