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Rat myocardial cell separation culture method

A technology for the separation and cultivation of cardiomyocytes, which is applied in the field of separation and culture of rat cardiomyocytes, can solve the problems of long synchronous contraction time of cardiomyocytes and low purity of cardiomyocytes, and achieve the effect of low cost, simple method and strong operability

Inactive Publication Date: 2015-08-12
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

2. Use 10% FCS / DMEM (high sugar) to cultivate cardiomyocytes. This medium is also suitable for the growth of cardiac fibroblasts, so the purity of cardiomyocytes obtained is low
3. Cardiomyocytes cultured by traditional methods take a long time to achieve synchronous contraction, generally at least 3 days, which cannot well meet the experimental requirements

Method used

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Embodiment Construction

[0016] The present invention is further illustrated below by means of examples, but the present invention is not limited to the scope of the examples. For the experimental methods that do not specify specific conditions in the following examples, select according to conventional methods and conditions, or according to the product instructions. The reagents and raw materials used in the present invention are all known technologies, technologies well known to those skilled in the art, and are easily available in the market.

[0017] The invention provides a method for separating and culturing rat cardiomyocytes, which has the advantages of high survival rate of isolated and cultured neonatal rat cardiomyocytes, complete separation of cardiomyocytes and non-cardiomyocytes, rapid cell attachment, high purity, and rhythmic synchronous contraction It is an ideal primary culture method of cardiomyocytes, which is early, easy to operate, and reproducible: it can meet the requirements ...

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Abstract

The present invention discloses a rat myocardial cell separation culture method. According to the present invention, the myocardial cells being subjected to separation culture through the method have characteristics of high survival rate, complete myocardial cell and non-myocardial cell separation, rapid cell adherence, high purity, and early rhythmicity synchronized contraction time, and the method has characteristics of simple operation and good repeatability, is an ideal myocardial cell primary culture method, and can meet requirements of cardiovascular disease generation and development mechanisms, cardiovascular drug and cardiac tissue engineering researches, and a variety of physiological and biochemical experiments.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a method for separating and culturing rat cardiomyocytes. Background technique [0002] It plays an important role in the study of cardiomyocyte growth and development, physiology, metabolism, and pathology. Therefore, the primary culture technology of cardiomyocytes has been widely used in the research of the mechanism of cardiovascular diseases, cardiovascular drugs and cardiac tissue engineering. Common cardiomyocyte culture methods in this field are as follows: 1. Use the limbs of suckling rats to fix them on the operation panel, and take the heart. If this is the case, the operation opening will be large, and there will be a lot of bleeding, and a large amount of blood cells will easily be mixed in the cardiomyocytes. 2. Adopt 10% FCS / DMEM (high sugar) to cultivate cardiomyocytes, this medium is also suitable for the growth of cardiac fibroblasts, so the pu...

Claims

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Application Information

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IPC IPC(8): C12N5/077
Inventor 蔡维霞胡大海朱雄翔韩军涛郑朝官浩杨薛康王耘川计鹏
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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