Preparation method and application of a genetic marker for high intramuscular fat content in Baicheng chicken leg muscle

A technology of intramuscular fat and Baicheng oil chicken, applied in the field of poultry genetic markers

Inactive Publication Date: 2018-03-27
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the standards for measuring the quality of chicken meat come from people's taste, and there is no unified standard at present

Method used

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  • Preparation method and application of a genetic marker for high intramuscular fat content in Baicheng chicken leg muscle
  • Preparation method and application of a genetic marker for high intramuscular fat content in Baicheng chicken leg muscle
  • Preparation method and application of a genetic marker for high intramuscular fat content in Baicheng chicken leg muscle

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Embodiment Construction

[0033] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0034] The present invention selects 60 120-day-old Baicheng oil chickens (white feathers), half male and half female, detects the second exon polymorphism of H-FABP and the content of intramuscular fat (IMF) in leg muscles, and analyzes both The relationship between.

[0035] PCR primers

[0036] 5′-CGACAAGGCGACGGTGAA-3′(forward)

[0037] 5′-TGGGGCAGGAAGGAGTTT-3′(reverse)

[0038] 1) PCR reaction system:

[0039]

[0040] 2) PCR reaction program:

[0041]

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Abstract

The invention discloses a preparation method and application of a genetic marker with a high intramuscular fat content in the thigh muscle of Baicheng oil chicken. The invention selects 60 white feathers of Baicheng oil chicken aged 120 days, half male and half female, and detects H-FABP second Exon polymorphisms and the content of IMF in intramuscular fat in leg muscles, and the relationship between them was analyzed. The experiments of the present invention show that the genetic marker prepared by the method of the present invention selects Baicheng oily chicken with high IMF content in leg muscles.

Description

technical field [0001] The invention belongs to the technical field of poultry genetic markers, and relates to a preparation method and application of a genetic marker with high intramuscular fat content in the leg muscle of Baicheng oil chicken, in particular to a kind of high intramuscular fat content in the leg muscle of Baicheng oil chicken (white feather). Preparation and application of genetic markers for fat content. Background technique [0002] With the improvement of people's living standards, there are stricter requirements on the quality and taste of meat. Chicken is the main meat on the table, and the quality of chicken has increasingly become the focus of attention. Most of the standards for measuring the quality of chicken meat come from people's taste, and there is no unified standard at present. After analysis, the high intramuscular fat IMF in chicken is considered to be a main indicator affecting the meat quality traits of chicken and improving the tender...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
Inventor 王永惠晓红孟炜吐尔逊江库尔班
Owner TARIM UNIV
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