Preparation method and application of a genetic marker for high intramuscular fat content in Baicheng chicken leg muscle
A technology of intramuscular fat and Baicheng oil chicken, applied in the field of poultry genetic markers
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[0033] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.
[0034] The present invention selects 60 120-day-old Baicheng oil chickens (white feathers), half male and half female, detects the second exon polymorphism of H-FABP and the content of intramuscular fat (IMF) in leg muscles, and analyzes both The relationship between.
[0035] PCR primers
[0036] 5′-CGACAAGGCGACGGTGAA-3′(forward)
[0037] 5′-TGGGGCAGGAAGGAGTTT-3′(reverse)
[0038] 1) PCR reaction system:
[0039]
[0040] 2) PCR reaction program:
[0041]
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