A multiple detection method and database of bean plant pathogenic bacteria

A technology for multiple detection of plant pathogenic bacteria, applied in the measurement/testing of microorganisms, biochemical equipment and methods, monitoring of invasive species, etc., can solve the problem that there are no multiple detection methods for bean bacterial diseases, so as to improve the prevention of external harmful Bio-invasion capability, improvement of inspection and quarantine speed, important practical and technical effects

Inactive Publication Date: 2017-01-04
SHENZHEN ACAD OF INSPECTION & QUARANTINE +1
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AI Technical Summary

Problems solved by technology

However, there is no multiple detection method and corresponding standard protein fingerprint database specifically for soybean bacterial diseases.

Method used

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  • A multiple detection method and database of bean plant pathogenic bacteria
  • A multiple detection method and database of bean plant pathogenic bacteria
  • A multiple detection method and database of bean plant pathogenic bacteria

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Experimental program
Comparison scheme
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Embodiment

[0030] 1. Materials

[0031] 1. Tested strains

[0032] The supplied strains of this example are shown in Table 1.

[0033] Table 1 Conditions of donated strains

[0034] Strain number a strain name strain source b 470582 (ATCC11365) Pseduomonas savastanoi pv. phaseolicola 1 470575 (ICMP2452) Pseduomonas syringae pv. pisi 2 470513 (psg363) Pseduomonas savastanoi pv. glycinea 4 470543 (NCPPB3869) Pseduomonas syringae pv. syringae 3 470709 (ATCC43911) Xanthomonas axonopodis pv. glycines 1 470719 (ATCC49119) Xanthomonas axonopodis pv. phaseoli 1 470366 (ATCC7392) Curtobacterium flaccumfaciens pv.flaccumfaciens 1

[0035] a , the six-digit code is the strain number of the Plant Pathogenic Bacteria Preservation Bank of the Plant Inspection and Quarantine Laboratory of Shenzhen Entry-Exit Inspection and Quarantine Bureau, and the number in brackets represents the original code of the strain.

[0036] b ,...

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Abstract

The application discloses a multiple detection method and database of bean plant pathogenic bacteria. Concretely discloses the use of the database constructed by the application to control bean diseases such as bean sickle disease, bean bacterial wilt pathogen, pea bacterial blight, soybean bacterial blight, soybean bacterial brown spot, soybean spot blight, and bean bacterial blight. Seven-fold specific detection method for important plant pathogenic bacteria. The multiple detection method of this application can detect seven kinds of pathogenic bacteria on beans at the same time, which improves the detection speed and provides a fast, sensitive and high-throughput analysis and detection method for port inspection and quarantine departments, which can shorten the traditional detection time , improve the speed of customs clearance and reduce the cost of enterprises; at the same time, it provides technical support for the safe production, introduction and export of bean crops, improves the interception rate of imported bean crops and their products, and improves the ability to prevent the invasion of foreign pests, which is of great practicality and technical.

Description

technical field [0001] The application relates to the field of bean bacterial disease detection, in particular to a multiple detection method for bean plant pathogenic bacteria and a database used in the detection method. Background technique [0002] According to data from the General Administration of Customs of China, China imported 58.38 million tons of soybeans in 2012, 63.38 million tons in 2013, and 70.7 million tons in 2014. It can be seen that China's soybean imports exceed 80%. Important phytopathogenic bacteria on beans mainly include soybean bacterial blight (Pseudomonass avastanoi pv.glycinea), soybean bacterial brown spot (Pseudomonas syringae pv.syringae), soybean spot disease (Xanthomonas axonopodis pv.glycines), soybean bacterial wilt Curtobacterium flaccumfaciens pv. flaccumfaciens, Pseudomonas savastanoi pv. phaseolicola, Xanthomonas axonopodis pv. phaseoli and Pseudomonas syringae pv. pisi. Among them, Phytophthora wilt of bean, Phytophthora wilt of bean...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
CPCY02A90/40
Inventor 冯建军章桂明龙海史秀杰
Owner SHENZHEN ACAD OF INSPECTION & QUARANTINE
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