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A kind of paraffin section method of plant tissue

A technology of paraffin sectioning and tissue sectioning, which is applied in the field of plants, can solve the problems of concentration, time difference, production failure, numerous procedures and links, etc., to enhance the effect of sectioning and observation, reduce the use of reagents, and improve production efficiency. Effect

Active Publication Date: 2018-03-16
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Conventional procedures for making paraffin sections include steps such as fixation, dehydration, transparency, wax immersion, embedding, and section staining. There are many procedures and links involved, and the cycle is long.
In addition, the reagents, concentrations, and time used for different tissue materials are different, and problems such as slice bending and tissue fragmentation are prone to occur during the production process, resulting in failure of production
[0004] The reasons for the failure of the production can be traced back to many deficiencies in the production process of paraffin sections, including dehydration, transparency, wax dipping, embedding, sectioning, dewaxing and sealing, etc. If the dehydration is not complete, it will cause transparency and opacity. Thoroughly, so that the paraffin is not fully immersed in the tissue, resulting in tissue deformation, section failure, etc., so the whole paraffin section production process needs a lot of time to explore the processing time, reagent conditions, processing methods, etc.

Method used

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  • A kind of paraffin section method of plant tissue
  • A kind of paraffin section method of plant tissue
  • A kind of paraffin section method of plant tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1) Take mature buckwheat seeds, use double-sided blades to adopt a cross-cutting method, and follow the attached Figure 4 Cut at dotted line A or B to remove embryo and endosperm.

[0051]2) Soaking: Soak the buckwheat seed peel in warm water at 65-70°C for 5-10 minutes;

[0052] 3) Water-containing embedding: use filter paper to quickly absorb the water on the surface and inside of the soaked peel, so that the moisture content of the peel is kept between 20% and 40%, and then quickly transfer it, with the cut of the peel facing upward, and slowly immerse it in the container. In a metal embedding bottom mold with paraffin wax at 60-65°C; among them, the bottom mold is pre-filled with paraffin wax, and after being placed on ice for 15-25 seconds, the peel is immersed in it and then set up on the embedding bottom mold for embedding box, add appropriate melted paraffin again;

[0053] 4) Slicing: Slice with a common biological slicer, with a slice thickness of 10-16 μm;...

Embodiment 2

[0058] 1)~5) as described in embodiment 1

[0059] 6) The tissue sections were not stained with safranin and fast green, but were immersed in 50%, 75%, and 85% ethanol in turn for 2 minutes each, then placed in absolute ethanol for 10 minutes, and xylene for 10 minutes;

[0060] 7) As described in Example 1. (The obtained slice effect is as follows figure 2 , d=12μm)

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Abstract

The invention relates to the field of plants, in particular to a method for paraffin sectioning plant tissues. In this method, after the buckwheat peel is soaked in warm water, the peel tissue contains 20% to 40% water, and then the "water-containing" embedding method is used to wrap the water-containing tissue with paraffin. The "water-containing" embedding method can replace the conventional method. The steps of dehydration, transparency, and wax dipping of paraffin sections, and at the same time use a dissecting needle to peel off the paraffin instead of dewaxing steps, greatly reducing the time and operation of the sectioning process. The method cuts out buckwheat pericarp tissue slices with a thickness of 10-16 μm, the tissue is complete, each part is clearly displayed, and the slice and observation effects are obviously enhanced.

Description

technical field [0001] The invention relates to the field of plants, in particular to a method for paraffin sectioning plant tissues. Background technique [0002] Paraffin section is the most widely used method in conventional histological preparation techniques. It is mainly used to observe the morphology of cells and tissues. After being sealed, the prepared slides can be made into permanent slides and stored for a long time. [0003] Conventional procedures for making paraffin sections include steps such as fixation, dehydration, transparency, wax immersion, embedding, and section staining, involving many procedures and links and a long cycle. Moreover, the reagents, concentrations, and time used by different tissue materials are different, and problems such as slice bending and tissue fragmentation are prone to occur during the production process, resulting in failure of production. [0004] The reasons for the failure of the production can be traced back to many defic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30
Inventor 赵钢向达兵宋超万燕谭茂玲晏林宋月周鑫
Owner CHENGDU UNIV
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