A kind of paraffin section method of plant tissue
A technology of paraffin sectioning and tissue sectioning, which is applied in the field of plants, can solve the problems of concentration, time difference, production failure, numerous procedures and links, etc., to enhance the effect of sectioning and observation, reduce the use of reagents, and improve production efficiency. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0050] 1) Take mature buckwheat seeds, use double-sided blades to adopt a cross-cutting method, and follow the attached Figure 4 Cut at dotted line A or B to remove embryo and endosperm.
[0051]2) Soaking: Soak the buckwheat seed peel in warm water at 65-70°C for 5-10 minutes;
[0052] 3) Water-containing embedding: use filter paper to quickly absorb the water on the surface and inside of the soaked peel, so that the moisture content of the peel is kept between 20% and 40%, and then quickly transfer it, with the cut of the peel facing upward, and slowly immerse it in the container. In a metal embedding bottom mold with paraffin wax at 60-65°C; among them, the bottom mold is pre-filled with paraffin wax, and after being placed on ice for 15-25 seconds, the peel is immersed in it and then set up on the embedding bottom mold for embedding box, add appropriate melted paraffin again;
[0053] 4) Slicing: Slice with a common biological slicer, with a slice thickness of 10-16 μm;...
Embodiment 2
[0058] 1)~5) as described in embodiment 1
[0059] 6) The tissue sections were not stained with safranin and fast green, but were immersed in 50%, 75%, and 85% ethanol in turn for 2 minutes each, then placed in absolute ethanol for 10 minutes, and xylene for 10 minutes;
[0060] 7) As described in Example 1. (The obtained slice effect is as follows figure 2 , d=12μm)
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com