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Method for detecting single-nucleotide polymorphism of goat ATBF1 gene and application of goat ATBF1 gene

A single nucleotide polymorphism and detection method technology, applied in the field of animal molecular breeding, can solve the problems of lack of research and blank function research of gene loci, and achieve the effect of accurate detection and low cost

Inactive Publication Date: 2015-09-02
NORTHWEST A & F UNIV
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Problems solved by technology

[0009] At present, the research on the ATBF1 gene is mainly focused on the research on cancer-related aspects, and the research on the genetic variation of the ATBF1 gene in Chinese local goats is scarce, and the functional research on this gene locus is even blank.

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  • Method for detecting single-nucleotide polymorphism of goat ATBF1 gene and application of goat ATBF1 gene
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  • Method for detecting single-nucleotide polymorphism of goat ATBF1 gene and application of goat ATBF1 gene

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[0044] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the present invention.

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Abstract

The invention discloses a method for detecting the single-nucleotide polymorphism of a goat ATBF1 gene and the application of the goat ATBF1 gene. According to the method, the goat whole-genome DNA to be tested is taken as a template, the goat ATBF1 gene is amplified by use of the PCR technology, the PCR product is digested by use of a restriction enzyme MspI or HpaII, next, the agarose gel electrophoresis is performed, and the genotype of No.25748nt or No.163517nt SNP of the goat ATBF1 gene is identified according to the electrophoresis result; different genotypes of the No.25748nt and No.163517nt SNPs of the ATBF1 gene have remarkable relevance with the growth and development traits of the goat, and therefore, the ATBF1 gene is advantageous for quickly establishing a good goat genetic resource population.

Description

technical field [0001] The invention belongs to the field of animal molecular breeding, and relates to a method for detecting single nucleotide polymorphism (SNP) of an important functional gene and its application, in particular to a method for detecting the single nucleotide polymorphism of goat ATBF1 gene and its application in breeding. application. Background technique [0002] Animal breeding technology mainly includes conventional breeding technology based on phenotype and phenotypic value and molecular breeding technology based on DNA polymorphism. As an important part of the molecular breeding technology system, marker-assisted selection (MAS) breeding technology first detects DNA polymorphisms of important genes, then analyzes the correlation between DNA polymorphisms and genetic traits, and finally Trait selection based on DNA markers significantly associated with genetic traits. As a new technology born during the development of modern molecular biology technol...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6827C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 蓝贤勇张晓燕陈宏雷初朝徐铁山陶明亮
Owner NORTHWEST A & F UNIV
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