Method for preventing tobacco root-knot nematode by using entomopathogenic nematode dead body agent
A technology for entomopathogenic nematodes and tobacco root-knot nematodes, applied in the field of biological control of tobacco pests and diseases, can solve problems such as threats to human health and ecological environment damage, and achieve the effects of convenient storage, high safety, and long control period
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[0018] The invention relates to a method for controlling root-knot nematode of flue-cured tobacco by adopting the dead agent of the entomopathogenic nematode of the family Steineridae, wherein the Latin name of the entomopathogenic nematode of the family Steineridae is S. carpocapsae , referred to as EPN, the present invention comprises the following sequential steps:
[0019] 1. Preparation of entomopathogenic nematode nematodes
[0020] Trapping entomopathogenic nematodes: Take soil from the field to the laboratory, and put 4-5 mature larvae of the greater wax moth into the soil. The Latin name of the greater wax moth is Galleria mellonella , Let the entomopathogenic nematodes in the soil automatically infect the wax moth for a week, pay attention to turning the mixture of the soil and the wax moth during the period, and manually peel the cocoons every day, because the wax moth spins silk at any time to protect itself , after the death of the wax moth, the dead body of the...
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