Alcoholic extract of Schisandra chinensis and its application in the preparation of anti-hepatic fibrosis drugs

A technology of schisandra extract and schisandra alcohol, which is applied in the field of medicine

Inactive Publication Date: 2019-03-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no literature report that the alcohol extract of Schisandra chinensis has the effect of anti-hepatic fibrosis

Method used

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  • Alcoholic extract of Schisandra chinensis and its application in the preparation of anti-hepatic fibrosis drugs
  • Alcoholic extract of Schisandra chinensis and its application in the preparation of anti-hepatic fibrosis drugs
  • Alcoholic extract of Schisandra chinensis and its application in the preparation of anti-hepatic fibrosis drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Preparation of Schisandra Alcoholic Extract 1

[0040] (1) Preparation of the extract: after crushing the schisandra medicinal material, put it into an extraction tank, namely 80% ethanol, the extraction temperature is 60° C., the amount of ethanol is 5 times, the number of extractions is 3 times, and the extraction time is 1 hour, and the extracts are combined;

[0041] (2) Refining and concentrating: the above-mentioned extract is filtered and concentrated to an alcohol-free state;

[0042] (3) Ethyl acetate extraction, concentration: the concentrated solution was diluted with water, followed by extraction with ethyl acetate once, the combined extracts were concentrated to a small volume.

[0043] (4) Purification: silica gel sample, fast normal phase column chromatography. The sample volume ratio is 5:1; the sample flow rate is 1ml min -1 ; Adsorption time is 2h; respectively first with water, 15% to remove impurities. Use petroleum ether ethyl acetate ...

Embodiment 2

[0044] Example 2: Preparation of Schisandra Alcoholic Extract 2

[0045] (1) Preparation of extraction liquid: After the schisandra medicinal material is crushed, put it into an extraction tank, that is, 95% ethanol, the extraction temperature is 80° C., the amount of ethanol is 30 times, the number of extractions is 2 times, and the extraction time is 2 hours, and the extraction liquid is combined;

[0046] (2) Refining and concentrating: the above-mentioned extract is filtered and concentrated to an alcohol-free state;

[0047] (3) Ethyl acetate extraction, concentration: the concentrated solution was diluted with water, extracted 5 times with ethyl acetate successively, the extracts were combined and concentrated to a small volume.

[0048] (4) Purification: silica gel sample, fast normal phase column chromatography. The sample volume ratio is 25:1; the sample flow rate is 4ml min -1 ; Adsorption time is 8h; first remove impurities with water and 15%. Use petroleum ether...

Embodiment 3

[0049] Example 3: Therapeutic Effects of Alcoholic Extract of Schisandra Fructus on Common Bile Duct Ligation Liver Fibrosis Model Rats

[0050] Liver fibrosis experiment with common bile duct ligation (Xu Shuyun, Bian Rulian, Chen Xiu. Pharmacological Experimental Methodology, 3rd Edition [M]. Beijing: People's Medical Publishing House, 2002: 807). The specific method is as follows:

[0051] SD rats, male and female, 6-8 weeks old, weighing 200±10 g, were provided by the Experimental Animal Center of the Second Military Medical University of the Chinese People's Liberation Army.

[0052] Breeding room temperature 18-22 ℃, relative humidity 60%-75%, artificial mixed feed feeding.

[0053] Randomly grouped, 10 rats in each group: sham operation group, model group, administration group (Schisandra chinensis alcohol extract 1, 80mg / kg·d), in the sham operation group, only abdominal incision was made, common bile duct was separated, and common bile duct was not performed. Ligate...

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Abstract

The invention relates to the technical field of a medicine. The invention provides a schisandra chinensis alcohol extract, an extraction method thereof, and an application of the schisandra chinensis alcohol extract to prepare an anti-hepatic fibrosis medicine. The invention provides a novel medical application of the schisandra chinensis alcohol extract. According to pharmacodynamics study, it is shown that the schisandra chinensis alcohol extract can obviously lower the concentrations of ALT and AST in serum of a mouse, improves the symptom of hepatic fibrosis, and has excellent anti-hepatic fibrosis activity. Therefore, the anti-hepatic fibrosis is applicable to prepare the anti-hepatic fibrosis medicine or a health-care food.

Description

Technical field: [0001] The invention relates to the technical field of medicine, in particular to the application of Schisandra chinensis alcohol extract in the preparation of anti-hepatic fibrosis medicine or health food. Background technique: [0002] Hepatitis, liver fibrosis, liver cirrhosis and liver cancer are common clinical diseases and frequently-occurring diseases, which seriously threaten people's health and life. Hepatitis is a worldwide liver disease. At present, there are about 300 million hepatitis patients in the world. There are more than 100 million people infected with hepatitis B virus in my country. Among them, about 300,000 to 400,000 people develop into liver cancer after liver fibrosis. In addition, the chemical poisons or toxin-induced liver damage caused by environmental pollution in China, as well as the alcoholic liver disease caused by the increase in the number of alcoholics, and the number of patients with chronic liver disease continue to ris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/79A61K36/57A61P1/16A23L33/105
Inventor 张海张国庆柴逸峰
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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