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A kind of method that utilizes Acinetobacter to increase the production of bacterial curdlan

A technology of Acinetobacter ruckeri and bacteria, applied in the field of increasing the output of curdlan gum, can solve the problem of high price of curdlan gum, and achieve the effect of increasing the yield of rubber

Active Publication Date: 2018-05-15
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of curdlan gum is still relatively high at present, increasing the output of curdlan gum and reducing production costs are the main direction of curdlan fermentation production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Acinetobacter ruckeri CGMCC 1.3778 was selected and purchased from China General Microorganism Culture Collection Center (CGMCC). Get a ring of bacteria from the slant of Acinetobacter ruckeri and inoculate it into a 250ml Erlenmeyer flask with 50ml seed culture medium, and cultivate it by shaking at 28°C and 200rpm for 20h, and use it as a seed solution; wherein, the composition of the medium (g / L) is : Peptone 10g, yeast extract 2g and MgSO 4 ·7H 2 O 1g;

[0023] (2) The curdlan-producing fungus Rhizobium ATCC 31750 was selected and purchased from the American ATCC Culture Collection. Take a ring of strains from the rhizobia slant and inoculate them into a 250ml Erlenmeyer flask containing 50ml of seed medium, and culture them with shaking at 28°C for 20 hours at a speed of 150rpm to obtain curdlan gum-producing bacteria seed liquid; wherein, the composition of the medium Same as step (1);

[0024] (3) the seed liquid of Acinetobacter ruckeri prepared in step ...

Embodiment 2

[0028] (1) Acinetobacter ruckeri CGMCC 1.3778 was selected and purchased from China General Microorganism Culture Collection Center (CGMCC). Get a ring of bacteria from the slant of Acinetobacter ruckeri and inoculate it into a 250ml Erlenmeyer flask with 50ml of seed medium, and cultivate it by shaking at 32°C and 150rpm for 22h, and use it as a seed solution; wherein, the composition of the medium is the same as in Example 1;

[0029] (2) The curdlan-producing bacterium Agrobacterium ATCC 31749 was selected and purchased from the American ATCC Culture Collection. Take a ring of bacteria from the slant of Agrobacterium and inoculate it into a 250ml Erlenmeyer flask with 50ml of seed medium, shake and culture at 32°C for 16h, with a rotation speed of 240rpm, to obtain the seed liquid of curdlan producing bacteria; wherein, the composition of the medium Same as step (1);

[0030] (3) The seed solution of Acinetobacter ruckeri prepared in step (1) and the seed solution of curdl...

Embodiment 3

[0034] (1) Acinetobacter ruckeri CGMCC 1.3778 was selected and purchased from China General Microorganism Culture Collection Center (CGMCC). Get a ring of bacteria from the slant of Acinetobacter ruckeri and inoculate it into a 250ml Erlenmeyer flask with 50ml of seed culture medium, and cultivate it by shaking at 30°C and 240rpm for 24h, and use it as a seed solution; wherein, the composition of the culture medium is the same as in Example 1;

[0035] (2) The curdlan-producing fungus Rhizobium ATCC 31750 was selected and purchased from the American ATCC Culture Collection. Take a ring of bacteria from the rhizobia slant and inoculate it into a 250ml Erlenmeyer flask with 50ml of seed medium, and culture it at 30°C for 20 hours with shaking at a speed of 200rpm to obtain the curdlan producing bacteria seed solution; wherein, the composition of the medium Same as step (1);

[0036] (3) The seed solution of Acinetobacter ruckeri prepared in step (1) and the seed solution of cur...

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Abstract

The invention relates to a method for increasing the yield of curdlan by using Acinetobacter, which comprises: co-cultivating Acinetobacter ruckeri and curdlan-producing bacteria to obtain the product. The method of the invention improves the rubber yield of the curdlan gum-producing bacteria, and provides a new method for microbial fermentation to produce the curdlan gum.

Description

technical field [0001] The invention belongs to the field of methods for increasing the yield of curdlan, in particular to a method for increasing the yield of curdlan by using Acinetobacter. Background technique [0002] Curdlan, also known as thermal gel, is a microbial exopolysaccharide mainly produced by bacteria of the genus Agrobacterium sp. and Rhizobium sp. Curdlan gum molecule is a linear molecule composed of glucose structural units connected by β-1,3 glycosidic bonds, without branches, and the relative molecular mass is about 5.3×10 4 ~2.0×10 6 . [0003] Curdlan gum has unique properties such as rheology, thermal gelation and simple structure, and can be widely used in food, biomedicine and other fields. Curdlan gum has the characteristic of heating and solidifying into gel, and the formed gel can be divided into low-grade gel and high-grade gel. Heat the aqueous dispersion of curdlan gum to 55-65°C and then lower the temperature to form a thermally reversibl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P19/04C12R1/01C12R1/41
Inventor 杨雪霞刘毅超付雅欣步国建
Owner DONGHUA UNIV
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