A rice stigma-specific strong promoter ossti1

A technology with strong promoter and stigma, applied in the fields of biotechnology and plant genetic engineering, can solve the problem of not being able to meet the needs of specific expression promoters well, and achieve the effects of improving reproductive characteristics, increasing metabolic burden, and improving reproductive performance.

Active Publication Date: 2018-07-31
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing methods for obtaining promoters all have their own problems more or less
Moreover, judging from the current relevant reports, the promoters obtained by the existing methods cannot well meet people's needs for specific expression promoters
[0006] Moreover, for some special plant parts, such as the stigma, there are no relevant reports on specific strong promoters for these parts

Method used

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  • A rice stigma-specific strong promoter ossti1
  • A rice stigma-specific strong promoter ossti1
  • A rice stigma-specific strong promoter ossti1

Examples

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Comparison scheme
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Embodiment Construction

[0036] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0037] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0038] Acquisition of OsSti1 promoter containing restriction site

[0039] Step 1. Design of primers

[0040] According to the whole genome sequence of rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice OsSti1 gene, and the enzyme cutting sites of the primers were designed according to the characteristics of the selected vector and target gene.

[0041] ...

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Abstract

The invention discloses a rice stigma-specific strong promoter OsSti 1 and its application. The promoter of the invention is based on the sequences in SEQ ID No: 1 and 2 in the sequence list and is extended on the basis. The present invention includes various variants of the above two sequences, especially the sequence with promoter function in the various variants. The invention also provides an expression cassette and a plant expression vector containing the rice stigma-specific strong promoter OsSti 1 and applies it to plant genetic engineering. In addition, the present invention also provides a set of primer pairs specially used for amplifying the promoter of the present invention from Nipponbare rice. The promoter of the invention has important theoretical and practical significance for the research on the molecular mechanism related to rice reproduction.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a rice stigma-specific expression promoter OsSti1 and its application. Background technique [0002] The regulation of gene expression is a multi-level complex process, controlled by different regulatory factors, and it is also realized at the multi-stage level. Although gene expression is a multi-level regulatory system in higher organisms, regulation at the transcriptional level is the most critical link. As an important regulatory element at the transcription level, the promoter is the final target of many transcription factors and RNA polymerases. Therefore, it is of great significance to study the structure, function and mode of action of the promoter in depth (Xu et al., 2011; yamamoto et al., 2011). [0003] Researching and improving crops by means of molecular biology and genetic engineering has good ap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/82A01H5/00A01H6/46
Inventor 魏鹏程杨剑波李莉秦瑞英李浩李娟杨亚春许蓉芳马卉
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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