In vitro maturation medium and application of human oocytes containing endothelin-1

Abstract

The invention provides a human oocyte in vitro maturation culture solution containing Endothelin-1. The human oocyte in vitro maturation culture solution comprises liquid A and liquid B, wherein the liquid A comprises ET-1, r-FSH and 10% SSS, the liquid B comprises ET-1, r-FSH, hCG and 10% SSS, and solvent is conventional basic culture solutions. Researches show that the culture solution and culture method are capable of promoting human oocyte in vitro maturation especially cytoplasmic maturation and can increase embryo development potential after human oocyte in vitro fertilization. The culture solution aiming at the current situation that development imbalance of cytoplast and cell nucleus during immature human oocyte in vitro culture affects embryo in vitro development potential is developed by utilizing the biological function and effect of ET-1 in cumulus cells. The culture solution has the advantages that the culture solution is applicable to human oocyte in vitro culture, an IVM process can be optimized favorably, and the culture solution is worthy of popularization and application.

Description

technical field

[0001] The invention belongs to the biomedical technical field of reproductive medicine, and relates to an in vitro maturation culture medium for human oocytes containing Endothelin-1 (ET-1) and its application in in vitro maturation and cultivation of immature human oocytes. Background technique

[0002] Human oocyte maturation in vitro (in vitro maturation, IVM) refers to the development of immature oocytes in follicles into mature second meiotic metaphase (MII) oocytes through in vitro culture, fertilization and division into embryos And transplant the technology to obtain pregnancy. Compared with conventional in vitro fertilization-embryo transfer (in vitro fertilization-embryotransfer), IVM can reduce the cost of high-dose ovulation-stimulating drugs, save time for medical treatment, and more importantly, avoid the side effects of drugs to stimulate ovaries. In some fields of reproductive medicine, IVM has an irreplaceable role, such as: IVM for patient...

Images