Method for quickly and synchronously detecting tomato yellow leaf curl virus and accompanying China tomato yellow leaf curl virus satellite

Abstract

The invention provides a method for quickly and synchronously detecting two virus components, including the tomato yellow leaf curl virus and the accompanying China tomato yellow leaf curl virus satellite, on tomatoes, and belongs to the field of plant protection. According to the nucleotide sequence of the tomato yellow leaf curl virus and the nucleotide sequence of the China tomato yellow leaf curl virus satellite, two pairs of primers are designed, namely TYmF / TYmR and TycnbF / TycnbR, wherein the TYmF / TYmR combination is used for detecting the tomato yellow leaf curl virus, and the TycnbF / TycnbR combination is sued for detecting the China tomato yellow leaf curl virus satellite. After total DNAs are extracted from a sample, only one time of PCR is needed to achieve detection of the two components including the tomato yellow leaf curl virus and the accompanying China tomato yellow leaf curl virus satellite. The method solves the problems existing in traditional biology, serology, electron microscopy observation and other methods. Compared with the traditional method that a virus is detected through one-time PCR, the detection method reduces cost, saves time and is a special, sensitive, economical and convenient method.

Description

technical field [0001] The invention relates to a method for synchronous detection of tomato yellow leaf curl virus and its accompanying satellite (Chinese tomato yellow leaf curl virus satellite), which belongs to the field of plant protection. Background technique [0002] Tomato yellow leaf curl virus (Tomato yellow leaf curl virus, TYLCV) is an important virus disease on tomato. In recent years, the tomato yellow leaf curl disease caused by it has occurred in a large area in my country's tomato producing areas. The tomato production caused serious losses, among which more than 5,000 mu of tomatoes in Wenzhou, Zhejiang were destroyed. Due to the rapid spread of the virus, severe damage, and difficulty in control, it has become one of the main limiting factors in current tomato production in my country. In terms of classification, tomato yellow leaf curl virus belongs to Geminiviridae and Begomovirus, and its transmission medium is Bemisia tabaci. The virus genome is a sin...

AI Technical Summary

Problems solved by technology

Compared with TYLCV infection alone, TYLCV+TYLCCNB infection will cause more severe symptoms, but the phenotypes of the two are difficult to distinguish in the early stage. At the same time, the two virus types have similar particle shapes and the same transmission media. and similar genome structure, which also brings great difficulties to its detection and identification

[0006] At present, the methods commonly used in the identification and identification of plant viruses include: biological inoculation, serological detection, electron microscope observation and molecular biological methods. Among these methods, some traditional detection methods are not suitable for the rapid identification of this new virus type. Detection, such as electron microscope observation (complex operation, similar size and shape of virus particles), biological inoculation identification (same mediator, long cycle), serological detection (serum needs to be prepared, detection is affected by protein specificity and expression abundance)

At present, molecular biology methods are widely used in the detection of this type of virus. Since this is a new type of virus, there is no method for simultaneous detection of two components using PCR.

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