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Novel diterpenoid alcohol, as well as preparation method and application thereof

A new type of diterpene alcohol technology, applied in the field of preparation of new diterpene alcohols, can solve the problems of extracting monomer compounds that have not been reported yet, and achieve good anti-tumor activity, good inhibitory activity, and rich structure types

Inactive Publication Date: 2015-11-18
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural products have the characteristics of diverse structures, but no monomeric compounds with good biological activity have been isolated from Tongjingcao

Method used

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  • Novel diterpenoid alcohol, as well as preparation method and application thereof
  • Novel diterpenoid alcohol, as well as preparation method and application thereof
  • Novel diterpenoid alcohol, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 compound

[0029] Weigh 1.5kg of the dried whole herb, extract with 80% ethanol under reflux for 2 hours, the volume ratio of 80% ethanol to Herba Tongjing is 15:1, collect the extract, and add 10 times the volume of 80% ethanol to the remaining samples for reflux extraction for 1 hour , collect the extract; combine the two extracts, filter, combine the filtrate, concentrate under reduced pressure to 2-5 times the volume (v / w), centrifuge at 4000rpm for 5 minutes, and the upper layer is sequentially washed with an equal volume of cyclohexane and chloroform , ethyl acetate, and n-butanol are distributed and extracted, the solvent is recovered, and the extracts of cyclohexane, chloroform, ethyl acetate, and n-butanol are obtained simultaneously.

[0030] The obtained chloroform extract was 20.6g, and a mixed solvent of chloroform-methanol (1:1, V / V) was added to dissolve the extract, and 30g of silica gel was added to mix well, and then p...

Embodiment 2

[0043] Activity test of embodiment 2 compound

[0044] Experimental Materials

[0045] RPMI-1640 medium, calf serum; thiazolyl blue (MTT), dimethyl sulfoxide; penicillin, streptomycin. HL-60 and HO-8910 cell lines were provided by Shanghai Cell Institute of Chinese Academy of Sciences.

[0046] The experimental drug was made by the above method, and the purity was 99.5%.

[0047] experimental method

[0048] HL-60 cells and ovarian cancer cells HO-8910 were cultured in RPMI-1640 medium containing 10% calf serum at 37°C and 5% CO 2 Under normal subculture conditions. Take the cells in the logarithmic growth phase, and use 2×10 5 Cell density per ml was inoculated in a 96-well culture plate, 200 μl per well. The blank control group was added with the same amount of medium without inoculating cells. Each group was added with different concentrations of aspirin B, and the control group was added with 0.5% DMSO, and cultured for 48 hours. Add 50 μl of MTT solution (5 mg / ml)...

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Abstract

The invention relates to a kaurane type diterpenoid alcoholic compound, as well as a preparation method and application thereof. According to the preparation method, kaurane type diterpenoid alcohol having a new structure is extracted and separated from aleuritopteris argentea fee by adopting natural products and a chemical means, so that structures of diterpenoid compounds can be enriched. The preparation method is simple and controllable, and the compound has high purity. A bioactivity detection on the compound indicates that the compound has an excellent anti-tumor activity, provides a new thought to research and development of anti-tumor medicines, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and in particular relates to a novel diterpene alcohol, its preparation method and application. Background technique [0002] Silverback fern Aleuritopteris argentea (Gmel.) Fee, a plant of the genus Fern in the family Pteridaceae, also known as Tongjingcao, has the effects of promoting blood circulation, regulating menstruation, and relieving cough. Natural products are characterized by diverse structures, but no monomeric compounds with good biological activity have been isolated from T. chinensis. Contents of the invention [0003] The present invention provides a diterpene alcohol compound with a new structure, which is named as farinadin B, and also provides a method for extracting it from Pteris argentina and its application in the field of antitumor. [0004] The diterpene alcohol compound structure of the present invention is shown in formula one, and chemical formula C 20 h ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C29/86C07C35/37A61P35/00
CPCC07C29/86C07C35/37
Inventor 李建晨廖明丽哈静
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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