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Section position using method of low concentration NAA gel

A low-concentration, gel technology, applied in the field of plant biology, can solve the problems of loss of branches, high cost, and many technical steps, and achieve the effects of reducing water evaporation and easy operation.

Inactive Publication Date: 2015-12-09
钱夕华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A method for using a low-concentration NAA gel at a node position, characterized in that the method includes hormone mother solution preparation, working solution preparation, hormone gel preparation, cuttings, and dripping at axillary buds. The specific operation steps are as follows:

[0014] ① Hormone mother solution preparation: Accurately weigh 0.1g of NAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL NAA mother solution;

[0015] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:100 into a low-concentration hormone working solution with a concentration of 10mg / L;

[0016] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethyl cellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone working solution with a concentration of 10 mg / L in a water bath at 50 °C for later use;

[0017] Cutting: Insert the calendula branch into th...

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PUM

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Abstract

The invention discloses a section position using method of a low concentration NAA gel and belongs to the technical field of plant biology. The method comprises: firstly preparing 1mg / mL NNA mother liquor; diluting the mother liquor to 10 mg / L low concentration NAA working liquor; preparing low concentration NNA gel sealing liquor containing sodium carboxymethylcellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8% by using the working liquor; inserting branches into a culture medium; and dropwise adding the gel sealing liquor into axillary buds of healthy and robust branches.

Description

Technical field [0001] A low -concentration NAA gel is used in the field of plant biotechnology. Background technique [0002] Cutting reproduction is one of the common methods of plants, especially trees without any sexual reproduction. This method has many advantages such as low cost, fast -effectiveness, short period of time, short period of time, rich sources of branches used, and can maintain the excellent characteristics of the pro -proof.Different types of hormones are often used during cuttings, such as NAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, ethylene, B 9 , PP333, MH, etc., the addition of the above hormones will enable plants to take root, flowering, fruiting, etc. under the condition of body or departure, increase the number of roots, induces the axillary buds, increase the fruit rate, etc., and play in agricultural production.Mainly useful use.However, it is extremely easy to cause death due to long -term losses of water or nutrients during the breeding or...

Claims

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Application Information

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IPC IPC(8): A01N25/04A01N43/40A01P21/00A01G7/06A01G1/00
Inventor 钱夕华
Owner 钱夕华