Purifying device for pretreatment of Aspergillus flavus and method adopting same

Abstract

The invention belongs to the field of pretreatment equipment, and particularly relates to a purifying device for pretreatment of Aspergillus flavus and a method adopting the same. The purifying device comprises a purifying column and a liquid storage pipe, the purifying column is composed of a rubber sealing head and a plastic pipe, diameter of the lower end of the rubber sealing head is 12mm while that of the upper end of the same is 8mm, a liquid passing hole is formed in the center of the rubber sealing head, degreased cotton is filled between the outer side of the liquid passing hole and the inner wall of the rubber sealing head, the upper end of the rubber sealing head is inserted into the plastic pipe, and a leakproof quartz filter element piece, purifying filler and a sealing quartz filter element piece are sequentially arranged in the plastic pipe from bottom to top. The purifying device is simple in structure, low in manufacturing cost, needless of negative pressure suction in the process of purifying and stable in purifying performance, purifying speed can be controlled manually, and purifying effect can meet subsequent detection needs.

Description

technical field [0001] The invention belongs to the field of pretreatment equipment, and in particular relates to a purification device for pretreatment of Aspergillus flavus and a method using the purification device. Background technique [0002] Aflatoxins are a class of compounds with similar chemical structures, all of which are derivatives of dihydrofuranocoumarins, and the common ones with strong toxicity are B1, B2, G1, G2, etc. Aflatoxin is extremely toxic. When people ingest a large amount, acute poisoning will occur, including acute hepatitis, hemorrhagic necrosis, fatty degeneration of liver cells and hyperplasia of bile ducts. When ingested in a small amount continuously, it will cause chronic poisoning, growth disorder, fibrous lesions, and fibrous tissue proliferation. The carcinogenicity of aflatoxin also ranks first, and it is one of the strongest carcinogens known so far. Aspergillus flavus is commonly found in soil, animals and plants, and various nuts, ...

AI Technical Summary

Problems solved by technology

[0003] At present, in the detection method of Aspergillus flavus, the purification column used is an immunoaffinity column. The manufacturing method of this purification column is relatively complicated, affected by the activity of immune antibodies, the purification performance is unstable, and the price is extremely high, which increases the actual detection cost. , the poor stability also brings a lot of inconvenience to the work

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