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Cold sterilization method based on gold nanoparticles in cooperation with high voltage field plasma

A gold nanoparticle and plasma technology, applied in food science, food preservation, application, etc., can solve the problems of losing antibacterial effect, cumbersome operation, and prone to coagulation, so as to avoid safety problems, simple operation procedures, No toxic by-product effects

Active Publication Date: 2016-02-24
南京苏曼等离子工程研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although both gold nanoparticles and high-voltage electric field plasma have bactericidal effects, both methods have certain shortcomings.
Compared with silver nanoparticles, the antibacterial activity of gold nanoparticles is low, and it cannot meet the actual sterilization requirements when used alone, and the high concentration of gold nanoparticles is extremely unstable in the bacterial solution, prone to coagulation and loss of antibacterial effect ; The existing problems of the high-voltage electric field plasma sterilization technology are: 1) the modified atmosphere sealed packaging is necessary to achieve a good sterilization effect, the operation is cumbersome and the cost is high; In rainy and humid conditions, the required high voltage is often not achieved; 3) Under such a high voltage intensity, long-term use of the equipment will increase the temperature of the electrodes and insulating plates, making the equipment unable to work normally
Based on the above reasons, the technology is currently unable to meet the actual production needs

Method used

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  • Cold sterilization method based on gold nanoparticles in cooperation with high voltage field plasma
  • Cold sterilization method based on gold nanoparticles in cooperation with high voltage field plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Prepare a PVP solution with a mass concentration of 0.5%, take 4mL of gold nanoparticles with a concentration of 8nM in a centrifuge tube, centrifuge at 13000r / min for 10min, remove the supernatant, add 2mL of 0.5%PVP solution for resuspension, and react at room temperature for 30min , thus obtaining a PVP functionalized gold nanoparticle solution. Add an equal volume of Staphylococcus aureus culture solution to the PVP functionalized gold nanoparticle solution, and incubate at 37°C for 2 hours. The voltage intensity is 10kv / cm.

Embodiment 2

[0032] Prepare a PVP solution with a mass concentration of 1%; take 12 mL of gold nanoparticles with a concentration of 8 nM in a centrifuge tube, centrifuge at 13000 r / min for 10 min, remove the supernatant, add 3 mL of 1% PVP solution for resuspension, and react at room temperature for 30 min, thereby A PVP functionalized gold nanoparticle solution was obtained. Add an equal volume of Staphylococcus aureus culture solution to the PVP functionalized gold nanoparticle solution, and incubate at 37°C for 3 hours. The voltage intensity is 20kv / cm.

Embodiment 3

[0034] Prepare a PVP solution with a mass concentration of 1.5%; take 24 mL of gold nanoparticles with a concentration of 8 nM in a centrifuge tube, centrifuge at 13000 r / min for 10 min, remove the supernatant, add 3 mL of 1.5% PVP solution for resuspension, and react at room temperature for 30 min , thus obtaining a PVP functionalized gold nanoparticle solution. Add an equal volume of Staphylococcus aureus culture solution to the PVP functionalized gold nanoparticle solution, and incubate at 37°C for 4 hours. The voltage intensity is 400kv / cm.

[0035] Comparison group 1-1

[0036] Except not adopting plasma treatment, other conditions are the same as embodiment 1.

[0037] Comparison group 1-2

[0038] 1) Add 500 μL of physiological saline to 500 μL of bacterial culture solution, and incubate at 37° C. for 4 hours.

[0039] 2) The 24-well plate containing the bacteria is placed between the two electrodes for plasma treatment, the plasma treatment time is 20s, and the tre...

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Abstract

The invention relates to a cold sterilization method based on gold nanoparticles in cooperation with high voltage field plasma and belongs to the nanomaterial sterilization technology field. Concretely, polyvinylpyrrolidone (PVP) is employed to modify gold nanoparticles, PVP functional gold nanoparticles are prepared, gold-PVP and bacteria are mixed and cultured, then the mixture is placed between two electrodes of a high voltage field plasma generation device, and by utilization of surface plasma resonance direct influences of a high voltage field on the gold nanoparticles in the bacteria, sterilization effects are generated in cooperation. When the provided method is compared with methods of sterilization of simple gold nanoparticles and high voltage filed plasma sterilization, air regulation seal package is not needed, the sterilization time is short, the used voltage is low, good sterilization effects can be achieved only by utilization of a small amount of gold nanoparticles, the temperature does not change obviously during the treatment process, and the provided method has potential application values at the fresh food fresh-keeping package aspect.

Description

technical field [0001] The invention relates to a cold sterilization method based on gold nanoparticles and high-voltage electric field plasma, and belongs to the technical field of nanomaterial sterilization. Background technique [0002] In recent years, with the successive exposure of vicious food safety incidents in various places, foodborne pathogens have become a common problem faced by the whole world. These pathogenic bacteria can easily contaminate food during food processing, production, transportation, storage, etc., and cause food deterioration while secreting toxic substances, thereby causing hemorrhagic enteritis, hemolytic uremic syndrome, sepsis and other diseases. Therefore, how to effectively inhibit or even kill pathogenic bacteria in food has become a hot spot of current research. Gold nanoparticles are nanomaterials with a size between 1-100nm. Due to their unique surface effects, small size effects, quantum effects, and macroscopic quantum tunneling ef...

Claims

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Application Information

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IPC IPC(8): A23L3/015A23L3/00
CPCA23L3/00A23L3/0155
Inventor 严文静章建浩黄倩施益纯刘天泽闵翠翠张恒
Owner 南京苏曼等离子工程研究院有限公司
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