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SSR markers in close linkage to rice black-streaked dwarf resistant QTL on chromosome 9 and application thereof

A technology for rice black-streaked dwarf and black-streaked dwarf disease, applied in the field of agricultural science, can solve the problems of poor repeatability, time-consuming and labor-intensive, and speed up the breeding process of disease-resistant varieties, so as to speed up the breeding process, clear the position, and identify the phenotype The method is accurate and reliable

Active Publication Date: 2016-02-24
SUQIAN CHOOSAN SEED IND
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Problems solved by technology

[0009] The object of the present invention is to provide a QTL closely linked with rice black-streaked dwarf resistance on chromosome 9 for the shortcomings of rice black-streaked dwarf resistance identification stability, poor repeatability, time-consuming and labor-intensive in conventional breeding. SSR markers and their application, the method can predict the resistance of plants to rice black-streaked dwarf disease, simplify the selection method, and accelerate the breeding process of disease-resistant varieties

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  • SSR markers in close linkage to rice black-streaked dwarf resistant QTL on chromosome 9 and application thereof
  • SSR markers in close linkage to rice black-streaked dwarf resistant QTL on chromosome 9 and application thereof

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Embodiment Construction

[0031] The methods used in the following examples are conventional methods unless otherwise specified.

[0032] 1. Screening of resistance sources and construction of F2 population of Guangfujing / Yangnongjing 2:

[0033] In 2013, a total of 251 micro-core rice varieties from 15 provinces were collected for field induction identification of black-streaked dwarf resistance. The results showed that there were significant differences in the resistance of rice varieties to black-streaked dwarf in different provinces. The japonica rice variety Guangfu japonica rice in Jiangsu has good resistance to black-streaked dwarf disease, and the field incidence rate is only 1%, which belongs to the high level of resistance (Southwest Agricultural Journal, 2014, 27 (6): 2365-2369). Using rice black-streaked dwarf resistance source Guangfujaponica as the female parent, rice black-streaked dwarf variety Yangnongjing 2 as the male parent, the hybrid F1 was prepared, and a F2 segregation populatio...

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Abstract

The invention relates to SSR markers in close linkage to rice black-streaked dwarf resistant QTL on a chromosome 9 and application thereof. According to the invention, a filial generation F2 population of a rice black-streaked dwarf resistant variety Guangfu japonica rice and a susceptible variety Yangnong japonica rice No.2 is used as a mapping population, rice black-streaked dwarf resistance identification is carried out through a method of combining field natural identification and improved laodelphax striatellus antixenosis identification, and a disease resistance identification result and SSR marker data are subjected to linkage analysis, so that two SSR markers in close linkage to rice black-streaked dwarf resistant main effect QTL on the chromosome 9 are obtained. Through detecting banding pattern data of the SSR markers on the chromosome 9, the resistance of plants to rice black-streaked dwarf can be predicted, the selection efficiency of rice black-streaked dwarf resistant rice is improved greatly and a breeding process is accelerated.

Description

technical field [0001] The invention relates to an SSR marker closely linked with a rice black-streaked dwarf resistance QTL on chromosome 9 and an application thereof, belonging to the field of agricultural science and technology. Background technique [0002] Rice black-streaked dwarf disease is a viral disease caused by rice black-streaked dwarf virus (RBSDV), which is mainly transmitted by the striatellus striatellus. There are 28 species of hosts including hybrid rice, conventional rice, barley, wheat, corn, millet, sorghum, kangaroo, bluegrass, barngrass, barnyardgrass, and matang. Rice black-streaked dwarf virus infects corn and causes corn rough dwarf disease, and infects wheat to cause wheat dwarf disease. The pathogenic forms, hosts and symptoms, vector insects and disease transmission characteristics of these diseases are very similar. Rice black-streaked dwarf disease can occur at the rice seedling stage, tillering stage, and jointing stage. Due to different inf...

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 宋立胜王纪芝王玲
Owner SUQIAN CHOOSAN SEED IND
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