Phenylalanine detection kit

A technology for detecting kits and phenylalanine, which is applied in the field of kits, can solve problems such as danger, expensive equipment, and inability to use testing instruments, and achieve the effects of low cost and avoiding hidden dangers

Inactive Publication Date: 2016-03-30
SUZHOU SYM BIO LIFESCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the first two methods are commonly used, but the bacterial inhibition method takes a long time and can only be semi-quantitative.
At present, the fluorescent color detection kits used in China are all imported products, which are often used in conjunction with specific testing instruments, and cannot be used for other testing instruments. Moreover, the kits and supporting equipment are expensive, which is not conducive to newborns. The popularization of newborn screening is beyond the capacity of ordinary newborn disease screening centers. At the same time, the imported kit contains sodium azide as a preservative in the liquid preparation, which is dangerous to humans in actual operation.

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of embodiment 1 kit

[0050] The phenylalanine detection kit of the present invention is provided with succinic acid solution 3, ninhydrin solution 4, dipeptide solution (L-leucyl-L-alanine solution) 5, copper reagent 6, calibrator blood Tablet 7 and quality control product blood sheet 8.

[0051] Wherein, the preparation method of each component in the kit is as follows:

[0052] 1) Preparation of succinic acid solution 3 (1L)

[0053] Accurately weigh 141g of succinic acid with an analytical balance, put it into an appropriate amount of beaker, add 70% of the prepared total volume of purified water to dissolve, use sodium hydroxide powder to adjust the pH value to 5.7, add Proclin300, stir well, and use purified The volume of water was adjusted to 1 L to obtain a succinic acid solution, wherein the final volume concentration of Proclin300 was 0.6%.

[0054] Dispense the prepared succinic acid solution 3 into reagent bottles according to the specifie...

Embodiment 2

[0075] The preparation of embodiment 2 kit

[0076] The phenylalanine detection kit of the present invention is provided with succinic acid solution 3, ninhydrin solution 4, dipeptide solution (L-leucyl-L-alanine solution) 5, copper reagent 6, calibrator blood Tablet 7 and quality control product blood sheet 8.

[0077] Wherein, the preparation method of each component in the kit is as follows:

[0078] 1) Preparation of succinic acid solution 3 (1L)

[0079] Accurately weigh 142g of succinic acid with an analytical balance, put it into an appropriate amount of beaker, add 80% of the prepared total volume of purified water to dissolve, use sodium hydroxide powder to adjust the pH value to 5.8, add Proclin300, stir well, and use purified The volume of water was adjusted to 1 L to obtain a succinic acid solution, wherein the final volume concentration of Proclin300 was 0.4%.

[0080] Dispense the prepared succinic acid solution 3 into reagent bottles according to the specifie...

Embodiment 3

[0102] The preparation of embodiment 3 kits

[0103] The phenylalanine detection kit of the present invention is provided with succinic acid solution 3, ninhydrin solution 4, dipeptide solution (L-leucyl-L-alanine solution) 5, copper reagent 6, calibrator blood Tablet 7 and quality control product blood sheet 8.

[0104] Wherein, the preparation method of each component in the kit is as follows:

[0105] 1) Preparation of succinic acid solution 3 (1L)

[0106]Accurately weigh 141.5g of succinic acid with an analytical balance, put it into an appropriate amount of beaker, add 75% of the prepared total volume of purified water to dissolve, use sodium hydroxide powder to adjust the pH value to 5.9, add Proclin300, after stirring evenly, use The volume of purified water was adjusted to 1 L to obtain a succinic acid solution, wherein the final volume concentration of Proclin300 was 0.5%.

[0107] Dispense the prepared succinic acid solution 3 into reagent bottles according to th...

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Abstract

The present invention discloses a phenylalanine detection kit comprising: a succinic acid solution, a ninhydrin solution, a dipeptide solution, a copper reagent, a calibrator blood film and a quality control blood film, and wherein the dipeptide solution is an L-leucyl-L-alanine solution. The kit can be used for quantitative determination of the concentration of phenylalanine in a sample blood film phenylalanine, and has the advantages of being accurate, fast, low in cost, and the like.

Description

technical field [0001] The invention relates to a kit, in particular to a phenylalanine detection kit. Background technique [0002] Phenylalanine is a kind of α-amino acid, and the optical isomer with biological activity is L-phenylalanine (L-Phenylalanine). Phenylalanine is one of the essential amino acids for human body. L-phenylalanine can be irreversibly converted into L-tyrosine (L-Tyrosine) by the coenzyme tetrahydrobiopterin in the living body, and then continue to decompose and generate a small amount of phenylpyruvate through transamination, but congenital phenylpropanoid In patients with amino acid hydroxylase deficiency, phenylalanine cannot be hydroxylated to tyrosine, and the production of phenylpyruvate increases, and phenylpyruvate appears in blood and urine, leading to mental retardation, which is called phenylketonuria (PKU ). [0003] Since Guthrie in the United States established the bacterial inhibition method to measure the concentration of phenylala...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 颜克亮
Owner SUZHOU SYM BIO LIFESCI CO LTD
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