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Application of emetine in preparing medicine for treating or preventing herpes virus infection

A herpes virus infection, herpes virus technology, applied in the field of biomedicine, to achieve the effect of cytopathic effect inhibition and good anti-herpes virus activity

Inactive Publication Date: 2016-04-06
武汉胜达康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, drugs to treat or prevent herpes virus infection still need to be further developed

Method used

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  • Application of emetine in preparing medicine for treating or preventing herpes virus infection
  • Application of emetine in preparing medicine for treating or preventing herpes virus infection
  • Application of emetine in preparing medicine for treating or preventing herpes virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the detection of emetine anti-HSV-1 activity

[0036] A kind of application of emetine in the preparation treatment or prevention herpes virus infection medicine, its steps are:

[0037] 1. Experimental materials:

[0038] 1.1 Cells and viruses:

[0039] Vero cells were purchased from the American Type Culture Collection (ATCC). HSV-1 is the F strain (Ejercito, P.M., E.D.Kieff, and B.Roizman.1968.Characterization ofherpessimplexvirus strains differingintheireffectsonsocialbehaviourofinfected cells.J.Gen.Virol.2:357-364), preserved for ChinaVirusResourceandBio-informationCenter (ChinaVirusResourceandBio-informationCenter) Base was purchased from Biochempartner.

[0040] 1.2 Reagents:

[0041] DMEM medium and FBS were purchased from GIBCO Company; Alamarblue activity detection kit was purchased from Thermofisher Company.

[0042] 1.3 Experimental equipment:

[0043] The multi-label microplate reader and high-content cell analyzer were purchased from Pe...

Embodiment 2

[0060] Embodiment 2: the detection of emetine anti-HSV-2 activity

[0061] A kind of application of emetine in the preparation treatment or prevention herpes virus infection medicine, its steps are:

[0062] 1. Experimental materials

[0063] 1.1 Cells and viruses

[0064] Vero cells were purchased from the American Type Culture Collection (ATCC). HSV-2 is G strain (Ejercito PM, Kieff ED, Roizman B. Characterization of herpessimplex virus strains differing in their effect on social behavior of infected cells. JGen Virol. 1968; 2:357-64.), preserved by China Virus Resource and Information Center (China Virus Resource and Bio-information Center). Emetine was purchased from Biochempartner.

[0065] 1.2 Reagents

[0066]DMEM medium and FBS were purchased from GIBCO Company; Alamarblue activity detection kit was purchased from Thermofisher Company.

[0067] 1.3 Experimental Instruments

[0068] The multi-label microplate reader and high-content cell analyzer were purchased fr...

Embodiment 3

[0085] Embodiment 3: emetine inhibits the active detection of KSHV lytic phase replication

[0086] A kind of application of emetine in the preparation treatment or prevention herpes virus infection medicine, its steps are:

[0087] 1. Experimental materials:

[0088] 1.1 Cells and viruses:

[0089] iSLK.219 cells were donated by Professor Dr.DonGanem (MyoungJ,GanemD(2011) Generationofadoxycycline-inducibleKSHVproducercelllineofendotheliaorigin:Maintenanceoftightlatencywithefficientreactivationuponinduction.JournalofVirologicalMethods174:12-21), the cells contain the expression of recombinant virus r9Acycycline1KSHV. Systemic SLK cells (isolated from endothelial cells in gingival Kaposi's sarcoma tissue). In this cell, the RTA expression sequence was constructed into the pRetro-XTet-ON inducible expression system (Clontech, Mountainview, CA), and the expression of this system was regulated by doxycycline. In the presence of doxycycline, RTA expression to occur; Vero cells w...

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PUM

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Abstract

The invention discloses an application of emetine in preparing medicine for treating or preventing herpes virus infection. Safe and toxic-concentration-free emetine is selected and used for an anti-herpes virus experiment, the result shows that emetine has remarkable anti-herpes virus activity and is relevant to dose dependence, and the anti-herpes virus effect of emetine is displayed by restraining virus reproduction. It is shown through detection of herpes viruses of different sub-types that emetine has broad-spectrum anti-herpes virus activity, is high in selection index, can be developed into the medicine for treating herpes virus infection diseases, and has wide application prospects.

Description

technical field [0001] The invention relates to the field of biomedicine, and more specifically relates to the application of emetine or a pharmaceutically acceptable salt thereof in the preparation of medicines for treating or preventing herpes virus infection. Background technique [0002] Herpesviridae is a family of viruses with envelopes and double-stranded DNA genomes. Members of this family can widely infect animals and humans and induce corresponding diseases. There are currently eight herpes viruses that can infect humans, including: Herpes simplex virus-1 (HSV-1), Herpes simplex virus-2 (HSV-2), Varicella-Zoster Herpes virus (Varicellazostervirus, VZV), Epstein-Barrvirus (EBV), cytomegalovirus (Cytomegalovirus, CMV), human herpesvirus type VI (HHV-6), human herpesvirus type VII (HHV-7) and card Bordeaux herpes virus (Kaposi's sarcoma-associated herpesvirus, KSHV). According to the differences in genome sequence, structure and physical and chemical properties, He...

Claims

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Application Information

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IPC IPC(8): A61K31/4725A61P31/22
CPCA61K31/4725
Inventor 吴建国陈绪林邬开朗刘映乐谭秋萍刘芳
Owner 武汉胜达康生物科技有限公司
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