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Ginseng medium for increasing content of panaxoside and preparing method thereof

A technology of ginsenosides and culture medium, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high cost and low yield, etc.

Inactive Publication Date: 2016-04-13
黄庆梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The ability of ginseng to treat diseases and maintain health mainly depends on ginsenosides, but the cost of directly extracting ginsenosides from ginseng plants is very high, and the yield is low. It can indirectly protect wild ginseng plants, but the content of ginsenosides in ginseng callus plays a decisive role in whether callus can be used instead of plants to extract ginsenosides

Method used

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  • Ginseng medium for increasing content of panaxoside and preparing method thereof
  • Ginseng medium for increasing content of panaxoside and preparing method thereof
  • Ginseng medium for increasing content of panaxoside and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Prepare a ginseng culture medium with increased ginsenoside content.

[0017] According to the B shown in Table 1 5 The medium formula and the ginseng medium formula with increased ginsenoside content shown in Table 2 were used to prepare the ginseng medium with increased ginsenoside content according to the present invention.

[0018] Weigh B separately with a balance 5 The components in the medium, as well as NAA (naphthalene acetic acid), 2,4-D (2,4-dichlorophenoxyacetic acid), lanthanum nitrate, agar powder and white sugar, were placed in a triangular flask, and an appropriate amount of purified water was added. Stir to dissolve, adjust the volume to 1000mL, and adjust the pH value to 6.5 with HCL or KOH. Seal the triangular flask, place it in an autoclave, and sterilize at 121°C for 21 minutes. Then, in an ultra-clean workbench, divide the ginseng culture medium with increased ginsenoside content into tissue culture bottles, cool naturally, and wait fo...

Embodiment 2

[0023] Example 2: Test of the ginseng culture medium with increased ginsenoside content according to the present invention.

[0024] According to the B shown in Table 1 5 For the medium formulation and the ginseng medium formulation with increased ginsenoside content shown in Table 3, refer to the medium preparation method described in Example 1 to prepare ginseng medium with increased ginsenoside content for each test group and control group.

[0025] Table 3 Components and dosages of ginseng medium for increasing ginsenoside content.

[0026]

[0027] Select the ginseng callus that has no pollution, normal growth, no browning and vitrification from the induction medium, take out the callus in the ultra-clean bench, and cut the callus into about 1 cubic meter size with a sterile scalpel The callus pieces were inoculated into the prepared ginseng culture medium with increased ginsenoside content in each test group and control group prepared above, and each piece of explant...

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Abstract

The invention provides a ginseng medium for increasing the content of panaxoside, and belongs to the technical field of ginseng tissue culture. A medium B5 is used as a basic medium, and naphthylacetic acid (NAA), 2,4-D(2,4-dichlorphenoxyacetic acid), lanthanum nitrate, agar powder and white granulated sugar are added. The medium has the advantages of being capable of increasing the content of panaxoside in callus and promoting callus proliferation.

Description

technical field [0001] The invention belongs to the technical field of ginseng tissue culture, and in particular relates to a ginseng medium for increasing the content of ginsenosides, and also relates to a preparation method for the medium for increasing the content of ginsenosides. Background technique [0002] Ginseng is an angiosperm, dicotyledonous plant class, subclass Araliaceae, a perennial herb plant, growing in dense forests, mostly distributed in the deep mountains of Heilongjiang, Jilin, Liaoning and northern Hebei, containing more than 10 kinds of ginsenosides, and ginseng fast Alcohol, a variety of amino acids and vitamins, is a valuable Chinese herbal medicine. [0003] The ability of ginseng to treat diseases and maintain health mainly depends on ginsenosides, but the cost of extracting ginsenosides directly from ginseng plants is very high, and the yield is low. It can indirectly protect wild ginseng plants, but the content of ginsenosides in ginseng callus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 何志铿
Owner 黄庆梅
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