Density gradient centrifugal tube with position-adjustable porous diaphragm and application of centrifugal tube

A technology of density gradient centrifugation and porous diaphragm, which is applied to special-purpose bioreactors/fermenters, sterilization methods, biochemical cleaning devices, etc., and can solve the problems of fixed volume of separation liquid, narrow application range, and non-adjustability , achieve the effect of simple operation, simple structure and no dead angle, and improve the separation effect

Active Publication Date: 2016-05-11
武汉海吉力生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the existing problems that the volume of separation liquid that each centrifuge tube can hold is fixed, cannot be adjusted, and the scope of application is narrow

Method used

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  • Density gradient centrifugal tube with position-adjustable porous diaphragm and application of centrifugal tube
  • Density gradient centrifugal tube with position-adjustable porous diaphragm and application of centrifugal tube
  • Density gradient centrifugal tube with position-adjustable porous diaphragm and application of centrifugal tube

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Embodiment 1

[0036] This embodiment provides a figure 1 , figure 2 with image 3 The shown density gradient centrifuge tube with a position-adjustable porous diaphragm includes a centrifuge tube body 2, a centrifuge tube cover 1 is provided at the upper end of the centrifuge tube body 2, a diaphragm layer 3 is provided inside the centrifuge tube body 2, and a centrifuge tube body 2 is provided with a centrifuge tube body 2. The tube body 2 divides the centrifuge tube into the upper space for containing the sample and the lower space for pre-loading the separation liquid through the diaphragm layer 3. The inner wall of the centrifuge tube body 2 is provided with an internal thread 5, and the outer wall of the diaphragm layer 3 is provided with an external thread. The centrifuge tube The internal thread 5 of the body 2 cooperates with the external thread of the diaphragm layer 3, and the diaphragm layer 3 is rotated to move up and down through the thread structure, and the space volume at ...

Embodiment 2

[0042] In this embodiment, the separation of human peripheral blood lymphocytes is taken as an example, and the density gradient centrifuge tube with a position-adjustable porous diaphragm of the present invention is used for separation, which specifically includes the following steps:

[0043] 1) Take the sterile centrifuge tube body 2, and add 15 ml of human peripheral blood lymphocyte separation solution into it.

[0044] 2) Put the diaphragm layer 3 into the centrifuge tube body 2, match the external thread of the diaphragm layer 3 with the internal thread 5 of the centrifuge tube body 2, and adjust the position of the diaphragm layer 3 to the level of the lower part of the diaphragm layer 3 and the separation liquid by turning the knob.

[0045] Wherein, the position adjustment of the diaphragm layer 3 can be completed by the auxiliary plastic rod 7, the center of the upper surface of the diaphragm layer 3 is provided with a slit 6, the lower end of the auxiliary plastic r...

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Abstract

The invention provides a density gradient centrifugal tube with a position-adjustable porous diaphragm. The density gradient centrifugal tube comprises a centrifugal tube body, a centrifugal tube cover is arranged at the upper end of the centrifugal tube body, a diaphragm layer is arranged in the centrifugal tube body, an internal thread is formed in the inner wall of the centrifugal tube body, an external thread is formed in the outer wall of the diaphragm layer, the internal thread of the centrifugal tube body is matched with the external thread of the diaphragm layer, and multiple small pores are formed in the diaphragm layer. The centrifugal tube is divided into the portion for containing a separating solution and the portion for being added with a sample to be detected through the diaphragm layer, premixing of the sample and the separating solution is effectively avoided, sample pollution caused during centrifuging and sampling is reduced, and the quality of centrifugal separation is greatly improved; meanwhile, a diaphragm layer rotary knob can be made to adjust the diaphragm layer up and down according to differences of the types and volumes of samples to be treated, so that optimal separation solution volume exists at the lower end of the diaphragm layer, and the application range is widened.

Description

technical field [0001] The invention specifically relates to a density gradient centrifuge tube with a position-adjustable porous diaphragm and its application, which is mainly suitable for separating white blood cells, red blood cells, lymphocytes, stem cells in human or animal body fluids, and circulating tumor cells in the field of medical diagnosis. Background technique [0002] With the rapid development of biotechnology, density gradient centrifugation has become a common biotechnology method for cell separation and enrichment. The rise of medical and therapeutic applications has brought density gradient centrifugation out of the laboratory. [0003] Density gradient centrifugation is to use a certain medium to form a continuous or discontinuous density gradient in the centrifuge tube, place the cell suspension or body fluid on the top of the medium, and make the mixed sample pass through the density gradient layer through the action of gravity or centrifugal force fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12M1/12C12M1/00
CPCC12M23/06C12M23/38C12M29/04C12M47/04
Inventor 张志立郭骁赵珊珊赵平锋
Owner 武汉海吉力生物科技有限公司
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