Preparation method of deciduous tooth pulp mesenchymal stem cells

A technology for stem cells and deciduous teeth, applied in the field of stem cell preparation, can solve the problems of subsequent use interference, uncertain components of the culture system, complicated steps, etc., and achieve the effects of long time consumption, maintaining cell viability and complicated operation.

Inactive Publication Date: 2016-05-25
江苏赫柏慧康再生医疗技术研究院有限公司
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects of cumbersome steps and uncertain components of the culture system caused by the enzymatic digestion method of the existing deciduous dental pulp mesenchymal stem cells, and to provide another Preparation method of deciduous tooth pulp mesenchymal stem cells

Method used

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  • Preparation method of deciduous tooth pulp mesenchymal stem cells
  • Preparation method of deciduous tooth pulp mesenchymal stem cells
  • Preparation method of deciduous tooth pulp mesenchymal stem cells

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Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Such as figure 1 as shown,

[0033] 1. Isolation and expansion culture of deciduous dental pulp mesenchymal stem cells

[0034] (1) The left upper central incisors of a 6-year-old child in Wuxi Children's Hospital were collected through natural shedding or surgical extraction; the collected deciduous teeth must be complete with roots, healthy and free of caries, pulp inflammation, and pulp necrosis, etc. symptom;

[0035] (2) Wash the collected deciduous teeth for 5 to 10 minutes in a cleaning solution consisting of penicillin sodium for injection (800,000 units / bottle), acamycin sulfate injection (200,000 units / bottle), metronidazole chloride Sodium chloride injection (250ml, metronidazole 0.5g) was prepared. After cleaning, it was transferred to deciduous tooth preservation solution (DMEM / F12) and transported to the laboratory at 2-8°C. The time should not exceed 48 ~ 96h;

[0036] (3) In the ultra-clean workbench, perform secondary cleaning, disinfection and ster...

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Abstract

The invention discloses a preparation method of deciduous tooth pulp mesenchymal stem cells. The method comprises the following steps: chopping tooth pulp tissue blocks, and directly culturing in a mesenchymal stem cell serum-free culture medium. The method is simple, quick and economical, and can better keep the cell activity and reduce the possibility of pollution. In the culture process, almost no impurity is introduced, so that the mechanical damage and chemical damage to the cells are low. By adopting the serum-free culture system, the obtained mesenchymal stem cells can be directly used for clinical use or scientific research. The obtained stem cells have high bioactivity and stem performance, the positive index expression rate is up to 98% or above, and the negative index expression rate is lower than 2%.

Description

technical field [0001] The invention relates to a method for preparing stem cells, in particular to a method for preparing deciduous dental pulp mesenchymal stem cells. Background technique [0002] Stem cells are a type of self-replicating cells that can differentiate into various functional cells under certain conditions. According to the developmental stage of stem cells, they can be divided into embryonic stem cells and adult stem cells; embryonic stem cells are totipotent in development and can differentiate into all tissues and organs of adult animals, including germ cells; adult stem cells are those that exist in a differentiated tissue Undifferentiated cells in , which are capable of self-renewal and specialize to form the cells that make up this type of tissue. Mesenchymal stem cells are a kind of adult stem cells with self-replication ability and multi-lineage differentiation potential. They can continuously self-renew and transform into one or more cells that con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02
CPCC12N5/0664A01N1/0221C12N5/0037C12N2500/90C12N2509/10
Inventor 刘洪杨熙汪梦云
Owner 江苏赫柏慧康再生医疗技术研究院有限公司
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