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Detection method of melamine in milk and milk powder

A melamine and detection method technology, applied in the detection field, can solve the problems of inability to quickly screen, heavy detection workload, high price, etc., and achieve the effect of rapid screening, low-cost detection and accurate results in large quantities and low cost

Inactive Publication Date: 2016-06-01
河北伊诺光学科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, at home and abroad about the detection method of melamine, mainly contain high performance liquid chromatography (HPLC), gas chromatography, ion chromatography, gas chromatography-mass spectrometry (GC-MS) and liquid mass spectrometry (LC-MS) etc., although These methods can accurately detect melamine in milk and milk powder products, but there are problems such as complicated operation, long sample processing time, inability to quickly screen, heavy detection workload and high price

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] S11, the milk processing method is as follows:

[0035] Take 2ml of milk samples containing different concentrations of melamine (1, 5, 10, 50, 100, 500, 1000mg / kg) into 10ml centrifuge tubes, accurately add 4ml of absolute ethanol, shake and mix well to obtain a mixed solution. After the mixture was centrifuged in a centrifuge for 5 min, the upper layer was taken to clarify the extract.

[0036] The detection limit of this method is 1 mg / kg.

[0037] S12, preparation of sample coffee ring

[0038] Use a pipette to absorb the obtained clear extract, drop it on a metal substrate or a coated glass substrate, and evaporate it at room temperature to obtain a coffee ring formed by the sample to be tested.

[0039] S13. Confocal Microscopic Raman Spectroscopy Detection of Coffee Ring Substances

[0040] Place the coffee ring formed by the sample to be tested under the microscope of a confocal Raman microscope to observe the distribution of the coffee ring substance, and pe...

Embodiment 2

[0042] S21, the milk processing method is as follows:

[0043] Take 10ml of milk samples containing different concentrations of melamine (1, 5, 10, 50, 100, 500, 1000mg / kg) into a 25ml beaker. Prepare 1mol / L hydrochloric acid (diluted with distilled water or pure water), add an appropriate amount of hydrochloric acid to the milk sample, adjust the pH to 4.7, denature and precipitate the protein, shake and mix well to obtain a mixed solution. Take 5ml of the mixed solution and place it in a 10ml centrifuge tube. After centrifuging for 5min, take the upper layer to clarify the extract.

[0044] The detection limit of this method is 1 mg / kg.

[0045] S22, preparation of sample coffee ring

[0046] Use a pipette to absorb the obtained clear extract, drop it on a metal substrate or a coated glass substrate, and evaporate it at room temperature to obtain a coffee ring formed by the sample to be tested.

[0047] S23. Confocal Microscopic Raman Spectroscopy Detection of Coffee Ring...

Embodiment 3

[0050] S31. Milk powder is processed as follows:

[0051] Take 1g of milk powder sample into a 25ml beaker, accurately add 9ml of distilled water or pure water, and magnetically stir to obtain a solution. Add different concentrations of melamine stock solution into the dissolving solution, so that the concentration of melamine is 1, 5, 10, 50, 100, 500, 1000 mg / kg. Prepare 1mol / L hydrochloric acid (diluted with distilled water or pure water), add an appropriate amount of hydrochloric acid to the milk powder solution containing melamine, adjust the pH to 4.7, denature and precipitate the protein, shake and mix well to obtain a mixed solution. Take 5ml of the mixed solution and place it in a 10ml centrifuge tube, centrifuge for 5min, and take the upper layer to clarify the extract.

[0052] The detection limit of this method is 1 mg / kg.

[0053] S32, preparation of sample coffee ring

[0054] Use a pipette to absorb the obtained clear extract, drop it on a metal substrate or ...

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Abstract

The invention discloses a detection method of melamine in milk and milk powder. Separation and enrichment characteristics of coffee-ring effect and high resolution detection of confocal micro-raman spectroscopy are fully combined, and a new detection method of low-concentration melamine in milk and milk powder is provided. The new detection method is simple and shortcut and has advantages of low cost and accurate result. Only by normal evaporation process, low-cost detection can be realized. The method is beneficial to large-batch low-cost rapid screening. The invention provides a new technical means for detection of melamine in milk and milk powder products.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of melamine in milk and milk powder. Background technique [0002] Since the "Sanlu Infant Milk Powder Incident" in 2008, people have paid great attention to the safety of dairy products, but incidents of melamine in milk and milk powder still occur frequently and have been banned repeatedly. Therefore, there is an urgent need to develop simple and efficient detection methods to meet the increasingly stringent requirements for the detection of melamine in milk and milk powder products. At present, at home and abroad about the detection method of melamine, mainly contain high performance liquid chromatography (HPLC), gas chromatography, ion chromatography, gas chromatography-mass spectrometry (GC-MS) and liquid mass spectrometry (LC-MS) etc., although These methods can accurately detect melamine in milk and milk powder products, but there are problems such as complicated o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 陈达闫孟雨
Owner 河北伊诺光学科技股份有限公司
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