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Application of acrisorcin for preparing medicine for treating or preventing herpesvirus infections

A technology of herpes virus infection and acridine, which is applied in the direction of antiviral agents, pharmaceutical formulations, active ingredients of hydroxyl compounds, etc., to achieve the effect of broad-spectrum anti-herpes virus activity

Active Publication Date: 2016-06-15
佛山熠然生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, drugs to treat or prevent herpes virus infection still need to be further developed

Method used

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  • Application of acrisorcin for preparing medicine for treating or preventing herpesvirus infections
  • Application of acrisorcin for preparing medicine for treating or preventing herpesvirus infections
  • Application of acrisorcin for preparing medicine for treating or preventing herpesvirus infections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the detection of anti-HSV-1 activity of acridinosorcin

[0036] A kind of application of acridizocine in the preparation of medicine for treating or preventing herpes virus infection, the steps are:

[0037] 1. Experimental materials:

[0038] 1.1 Cells and viruses:

[0039] Vero cells were purchased from the American Type Culture Collection (ATCC). HSV-1 is F strain (Ejercito, P.M., E.D.Kieff, and B.Roizman.1968.Characterization ofherpessimplexvirus strains differingintheireffectsonsocialbehaviourofinfected cells.J.Gen.Virol.2:357-364), which is preserved by ChinaVirusResourceandBio-informationCenter Sorin was purchased from Biochempartner.

[0040] 1.2 Reagents:

[0041] DMEM medium and FBS were purchased from GIBCO Company; Alamarblue activity detection kit was purchased from Thermofisher Company.

[0042] 1.3 Experimental equipment:

[0043] The multi-label microplate reader and high-content cell analyzer were purchased from PerkinElmer; the cell...

Embodiment 2

[0060] Embodiment 2: the detection of the anti-HSV-2 activity of acridinosorcin

[0061] A kind of application of acridizocine in the preparation of medicine for treating or preventing herpes virus infection, the steps are:

[0062] 1. Experimental materials

[0063] 1.1 Cells, viruses

[0064] Vero cells were purchased from the American Type Culture Collection (ATCC). HSV-2 is G strain (Ejercito PM, Kieff ED, Roizman B. Characterization of herpessimplex virus strains differing in their effect on social behavior of infected cells. JGen Virol. 1968; 2:357-64.), preserved by China Virus Resource and Information Center (China Virus Resource and Bio-information Center). Acridosocine was purchased from Biochempartner.

[0065] 1.2 Reagents

[0066] DMEM medium and FBS were purchased from GIBCO Company; Alamarblue activity detection kit was purchased from Thermofisher Company.

[0067] 1.3 Experimental Instruments

[0068] The multi-label microplate reader and high-content cel...

Embodiment 3

[0085] Embodiment 3: acridinesorcin inhibits the active detection of KSHV lytic phase replication

[0086] A kind of application of acridizocine in the preparation of medicine for treating or preventing herpes virus infection, the steps are:

[0087] 1. Experimental materials:

[0088] 1.1 Cells and viruses:

[0089] iSLK.219 cells were donated by Professor Dr.DonGanem (MyoungJ,GanemD(2011) Generationofadoxycycline-inducibleKSHVproducercelllineofendotheliaorigin:Maintenanceoftightlatencywithefficientreactivationuponinduction.JournalofVirologicalMethods174:12-21), the cells contain the expression of recombinant virus r9Acycycline1KSHV. Systemic SLK cells (isolated from endothelial cells in gingival Kaposi's sarcoma tissue). In this cell, the RTA expression sequence was constructed into the pRetro-XTet-ON inducible expression system (Clontech, Mountainview, CA), and the expression of this system was regulated by doxycycline. In the presence of doxycycline, RTA Expression can ...

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Abstract

The invention discloses application of acrisorcin for preparing a medicine for treating or preventing herpesvirus infections. Safe acrisorcin with non-toxicity concentration is selected to be subjected to an anti-herpesvirus experiment; it is shown that the safe acrisorcin with non-toxicity concentration has the remarkable anti-herpesvirus activity and is in dose-dependent relevance in the anti-herpesvirus experiment and the acrisorcin has anti-herpesvirus effect by restraining virus reproduction. The acrisorcin has the broad-spectrum anti-herpesvirus activity through detection display of herpes viruses of different subtypes, the selection index is high, and the safe and efficient medicine with small side effects is provided for treating herpes viruses clinically. The acrisorcin can remarkably restrain production of infectious herpes virions and restrain reproduction of the herpes viruses, the medicine for treating herpesvirus infected diseases can be further developed, and the medicine has the wide application prospect.

Description

technical field [0001] The present invention relates to the field of biomedicine, and more specifically relates to the application of acridinosorcin or a pharmaceutically acceptable salt thereof in the preparation of medicines for treating or preventing herpes virus infection. Background technique [0002] Herpesviridae is a family of viruses with envelopes and double-stranded DNA genomes. Members of this family can widely infect animals and humans and induce corresponding diseases. There are currently eight herpes viruses that can infect humans, including: Herpes simplex virus-1 (HSV-1), Herpes simplex virus-2 (HSV-2), Varicella-Zoster Herpes virus (Varicellazostervirus, VZV), Epstein-Barrvirus (EBV), cytomegalovirus (Cytomegalovirus, CMV), human herpesvirus type VI (HHV-6), human herpesvirus type VII (HHV-7) and card Bordeaux herpes virus (Kaposi's sarcoma-associated herpesvirus, KSHV). According to the differences in genome sequence, structure and physical and chemical...

Claims

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Application Information

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IPC IPC(8): A61K31/473A61P31/22A61K31/05
CPCA61K31/05A61K31/473A61K2300/00
Inventor 吴建国陈绪林邬开朗刘映乐谭秋萍刘芳
Owner 佛山熠然生物科技有限公司
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