Method for building animal model with PHF 14 gene knockout-based acute kidney injury and renal fibrosis after injury

A technology for acute kidney injury and renal fibrosis, which can be used in compound screening/testing, drug combinations, pharmaceutical formulations, etc.

Active Publication Date: 2016-06-15
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

However, the relationship between the PHF14 gene and fibrosis-related diseases in adulthood has not yet been elucidated.
In order to study the role of this gene in the pathogenesis of renal tubulointe

Method used

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  • Method for building animal model with PHF 14 gene knockout-based acute kidney injury and renal fibrosis after injury
  • Method for building animal model with PHF 14 gene knockout-based acute kidney injury and renal fibrosis after injury
  • Method for building animal model with PHF 14 gene knockout-based acute kidney injury and renal fibrosis after injury

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Experimental program
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Embodiment 1

[0043] 1. Materials and methods

[0044] 1. Preparation and grouping of AKI animal models: IVC-grade adult C57BL / 6 wild-type mice aged 8 to 12 weeks, weighing 20-35 g, provided by JAX Laboratory in the United States, and bred in the Experimental Animal Center of Second Military Medical University. According to the random number table method, 20 animals were divided into 4 groups (A-D), 5 animals in each group. All animals in B-D group were given folic acid (dissolved in 0.3mol / L NaHCO 3 In this method, the final concentration of folic acid is 10g / L) intraperitoneal injection, the dose is 250mg / kg body weight, and the folic acid is provided by Dalian Meilun Company. Group A animals were given 0.3mol / L NaHCO 3 Intraperitoneal injection, dose 25mL / kg body weight. Blood was collected from the A-D group at 0, 2, 14 and 28 days after intraperitoneal administration, the mice were sacrificed, and the kidney tissues were collected.

[0045] 2. Production of PHF14 adult-specific ind...

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Abstract

The invention relates to the technical field of animal models and concretely, relates to a method for building an animal model with PHF 14 gene knockout-based acute kidney injury and renal fibrosis after injury. The invention builds a model of renal fibrosis after kidney injury under the genetic background of PHF 14 gene knockout under adult conditions and provides essential conditions for researching a use of a PHF 14 gene in fibrotic diseases. Compared with the existing PHF 14 knockout model, the PHF 14 gene knockout provided by the invention does not cause embryonic lethal. A test on adult-stage tamoxifen induced knockout of PHF 14 proves that the PHF 14 gene knockout does not cause embryonic lethal. Compared with a group without knockout, the group subjected to knockout has no substantial pathology of lung, liver and kidney. The method prevents systemic disease state-caused interference on following-up acute kidney injury model making and realizes individual research on use of the gene defect in renal fibrosis pathogenesis.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a method for establishing an animal model of PHF14 gene knockout combined with acute kidney injury and post-injury renal fibrosis. Background technique [0002] Acute kidney injury (acute kidney injury, AKI) is characterized clinically by a sharp decline in renal function within a few days. The main etiology is renal ischemia-reperfusion injury, sepsis, nephrotoxic drug attack and post-renal obstruction. Microscopic pathology of AKI usually manifests as renal tubular epithelial cell necrosis and shedding, tubular cast formation, and interstitial inflammatory cell infiltration. Both animal experiments and prospective clinical studies have found that the risk of developing chronic kidney disease (chronickidney disease CKD) in the future is greater than that of those who have not experienced AKI, even if the kidneys that have experienced AKI can recover short-term renal funct...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K45/00A61P13/12A01K67/02
CPCA01K67/02A61K45/00A61K49/0008
Inventor 毛志国杨博陈思秀陈正军
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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