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A method for the preparation of biodiesel by enhancing the microbial enrichment and lipid production of excess sludge

A technology for excess sludge and biodiesel, which is applied in the field of sludge recycling, can solve problems such as unsatisfactory efficiency, and achieve the effects of high added value and improved carbon source conversion rate.

Active Publication Date: 2020-02-14
TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Studies have shown that under the optimal pH and optimal C / N culture conditions, the improved acid-heat oil extraction method can greatly improve the production efficiency of the final biodiesel compared with the original sludge after fermentation until about the fifth day. The esterification efficiency and biodiesel production efficiency per unit mass of dry sludge reached 36.92% and 13.06%, respectively, which were 4.55 times and 8.7 times that of the original sludge. According to the above standard, oil extraction has more economic value”, but the conversion efficiency of the corresponding unit mass of carbon source into biodiesel is not satisfactory, only about 4%, and the cost of adding carbon source in the culture medium The proportion is extremely high. In order to further meet the technical and economic requirements of biodiesel synthesis, in addition to oil extraction methods, culture condition pH and C / N optimization measures, other measures still need to be taken to improve the carbon source conversion rate or further increase Production efficiency of biodiesel

Method used

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  • A method for the preparation of biodiesel by enhancing the microbial enrichment and lipid production of excess sludge
  • A method for the preparation of biodiesel by enhancing the microbial enrichment and lipid production of excess sludge
  • A method for the preparation of biodiesel by enhancing the microbial enrichment and lipid production of excess sludge

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Embodiment 1

[0038] The invention discloses a method for preparing biodiesel by strengthening the enrichment and fat production of excess sludge microorganisms, the method comprising the following steps:

[0039] (1) Put the remaining sludge into the culture medium, add FeCl 3 Post-stirring fermentation: The influent of the sewage treatment plant is mainly domestic sewage, and some of it is industrial wastewater. The SBR process is adopted. The water quality indicators of the sewage treatment plant are as follows: COD cr =245.1mg / L, BOD 5 =86.1mg / L, SS=128.5mg / L, NH 3 -N=18.0 mg / L, COD / N=13.7, BOD / N=4.8, TP=2.22 mg / L, TN=30.0 mg / L. In the examples, after the sludge was sampled, it was sedimented and concentrated at 4°C for 24 hours and then the supernatant was removed. The MLVSS (Mixed Liquor Volatile Suspended Solids) of the sludge after sedimentation and concentration was about 20g / L. The initial MLVSS after inoculation into the fermentation medium is about 2.0-3.0g / L.

[0040] The f...

Embodiment 2

[0057] The method for preparing biodiesel by using the same method as in Example 1 to strengthen the enrichment of excess sludge microorganisms to produce fat, the difference is that the concentration of Fe in the culture medium before fermentation is 0.1mmol / L (as Fe 3+ count).

Embodiment 3

[0059] The method for preparing biodiesel by using the same method as in Example 1 to strengthen the enrichment of excess sludge microorganisms to produce fat, the difference is that the concentration of Fe in the culture medium before fermentation is 0.25mmol / L (as Fe 3+ count).

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Abstract

The invention relates to a method for preparing biodiesel by enhancing microorganisms in excess sludge to enrich and produce grease. The method comprises the following steps of firstly, placing the excess sludge in a culture medium, adding FeCl3, and then stirring and fermenting; secondly, after the fermenting is ended, separating and performing cold-drying to obtain dry sludge powder; thirdly, extracting microorganism grease in the dry sludge powder obtained in the second step by adopting a homogenizing-acid heating-organic solvent method; fourthly, converting the microorganism grease by adopting a boron trifluoride methyl esterification method to obtain the biodiesel. Compared with the prior art, the method disclosed by the invention has the advantages of high carbon source conversion rate, excellent preparation efficiency of the biodiesel and low production cost, and provides a new recycling path with a high added value for treatment of the excess sludge.

Description

technical field [0001] The invention relates to the field of sludge resource utilization, in particular to a method for enhancing the enrichment and lipid production of excess sludge microorganisms to prepare biodiesel. Background technique [0002] In today's world, human's demand for fuel is increasing day by day, and conventional fuel mainly comes from non-renewable energy sources such as oil and natural gas. The world is facing a severe energy crisis. In response to this crisis, since 2008, the research on using biodiesel as a traditional fuel has become a hot spot in the field of energy research. New research shows that primary and secondary sludge contains lipids and thus serves as a potential feedstock for biodiesel production. However, under the normal operating conditions of sewage treatment plants, due to the influence of low-carbon and high-nitrogen conditions in the influent, microorganisms in excess sludge cannot effectively store carbon sources in their bodie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11C3/04C10L1/02
CPCC10L1/02C11C3/04Y02E50/10
Inventor 刘佳黄翔峰王一涵陆丽君沈怡
Owner TONGJI UNIV
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