A biofilm control method of water supply pipe network based on group sensing effect and its application

A technology of water supply pipe network and control method, which is applied in the directions of biochemical equipment and methods, cleaning methods and utensils, separation of microorganisms, etc. small amount of effect

Active Publication Date: 2021-10-22
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This article is currently the only journal paper on biofilm and group sensing effect found in Chinese journals, but this article mainly focuses on the prevention and control strategies of streptococcal disease, and the growth environment and alternative control strategies of biofilm in urban water supply systems They're all completely different and don't have any reference value

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  • A biofilm control method of water supply pipe network based on group sensing effect and its application
  • A biofilm control method of water supply pipe network based on group sensing effect and its application
  • A biofilm control method of water supply pipe network based on group sensing effect and its application

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Experimental program
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Effect test

Embodiment 1

[0046] A water supply pipe network biofilm control method based on group sensing effect, comprising the following steps:

[0047] A. Taking the cement-lined ductile iron pipe section with a diameter of 100mm and long-term stable water flow as the research object, the air-water erosion experiment was carried out. Collect the biofilm samples of the experimental pipe section before flushing and after the air-water flushing, add 1mL sterile water to make a suspension, and dilute in turn 10 1 、10 2 、10 3 、10 4 、10 5 times, take the original solution and 0.1mL of the dilution solution of each gradient concentration, and spread them on the R2A solid medium plate, mark and culture.

[0048] The R2A plate was cultured at 28°C for 1-7 days and observed on the plate. According to the shape and color of the colony, streak the R2A plate continuously, and separate and purify the microbial strains on the biofilm before and after flushing. Pick the isolated and purified strains from the...

Embodiment 2

[0058] A water supply pipe network biofilm control method based on group sensing effect, comprising the following steps:

[0059] A. Taking the cement-lined ductile iron pipe section with a diameter of 100mm and long-term stable water flow as the research object, the air-water erosion experiment was carried out. Collect the biofilm samples of the experimental pipe section before flushing and after the air-water flushing, add 1mL sterile water to make a suspension, and dilute in turn 10 1 、10 2 、10 3 、10 4 、10 5 times, take the original solution and 0.1mL of the dilution solution of each gradient concentration, and spread them on the R2A solid medium plate, mark and culture.

[0060] The R2A plate was cultured at 28°C for 1-7 days and observed on the plate. According to the shape and color of the colony, streak the R2A plate continuously, and separate and purify the microbial strains on the biofilm before and after flushing. Pick the isolated and purified strains from the...

Embodiment 3

[0069] A water supply pipe network biofilm control method based on group sensing effect, comprising the following steps:

[0070] A. Taking the cement-lined ductile iron pipe section with a diameter of 100mm and long-term stable water flow as the research object, the air-water erosion experiment was carried out. Collect the biofilm samples of the experimental pipe section before flushing and after the air-water flushing, add 1mL sterile water to make a suspension, and dilute in turn 10 1 、10 2 、10 3 、10 4 、10 5 times, take the original solution and 0.1mL of the dilution solution of each gradient concentration, and spread them on the R2A solid medium plate, mark and culture.

[0071] The R2A plate was cultured at 28°C for 1-7 days and observed on the plate. According to the shape and color of the colony, streak the R2A plate continuously, and separate and purify the microbial strains on the biofilm before and after flushing. Pick the isolated and purified strains from the...

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Abstract

The invention provides a biofilm control method of a water supply pipe network based on group sensing effect; the method comprises the following steps: A, separating, purifying and identifying the strains of the biofilm on the wall of the water supply pipe network before and after flushing; B 1. Measure the content of each component of the extracellular polymeric substance (EPS) of the bacterial strain on the water supply pipe network wall before and after scouring that is separated; The key signaling molecule; D. Exogenous addition of quorum effect inhibitors, and the optimal dosage of quorum effect inhibitors can be determined. The invention is a new method proposed aiming at the limited efficiency of traditional pipe network disinfection, flushing and upgrading water quality purification process to remove biofilm in water supply pipe network. The method of the invention is mainly aimed at strong adhesion to water supply pipe walls and resistance to scouring microorganisms, and is environmentally friendly, simple in process, low in cost, saves water resources, and has obvious strengthening effect on the traditional method for removing biofilm in pipe networks.

Description

technical field [0001] The invention relates to the technical field of urban water supply pipe network water quality control, in particular to a biofilm control method and application of water supply pipe network based on group sensing effect. Background technique [0002] As one of the common forms of microbial existence in urban water supply pipe networks, the existence of pipe network biofilm is the main cause of secondary pollution in the process of urban drinking water transmission and distribution. On the one hand, the active microorganisms on the biofilm cause deterioration of drinking water quality (turbidity, color, smell, etc.) Studies have found that 20% of total pipeline losses are due to microbial corrosion. On the other hand, microorganisms exist in the form of biofilm, which not only greatly enhances the adaptability of bacteria in drinking water to stressful environments such as high chlorine and oligonutrients, but also provides for the restorative regrowth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B08B9/032C12N1/02C12Q1/6869C12Q1/6806C12Q1/04
CPCB08B9/032C12N1/02C12Q1/6806C12Q1/6869
Inventor 白晓慧王杨张东张立尖朱斌童俊
Owner SHANGHAI JIAOTONG UNIV
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