Kit, method and application for detecting kappa light chain content

A kit and light chain technology, applied in the field of kits for detecting KAPPA light chain content, can solve the problems of inability to make a clear diagnosis, inaccurate quantification, and long detection time in atypical patients, and achieve a high degree of automation and high detection efficiency. , the effect of improving detection efficiency

Active Publication Date: 2019-04-30
潍坊三维生物工程集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former detection time is longer, the operation is more cumbersome, and it cannot make a definite diagnosis for atypical patients
Enzyme-linked immunoassay has an enzymatic amplification effect, and the quantification is not accurate enough, which limits its application

Method used

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  • Kit, method and application for detecting kappa light chain content
  • Kit, method and application for detecting kappa light chain content
  • Kit, method and application for detecting kappa light chain content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The kit for detecting the content of KAPPA light chain comprises: reagent R1, reagent R2 and KAPPA light chain calibrator.

[0059] Wherein, reagent R1 comprises the following components:

[0060]

[0061] Reagent R2 includes the following components:

[0062]

[0063]

[0064] KAPPA Light Chain Calibrators include the following components:

[0065]

[0066] According to the required concentration of the calibrator, add the corresponding amount of KAPPA light chain antigen to the mixture of other reagents above, and mix to obtain the KAPPA light chain calibrator. In this example, 5 different concentrations of KAPPA light chain calibrator were prepared, and the concentrations of the KAPPA light chain antigen were: 0mg / dL, 200mg / dL, 400mg / dL, 800mg / dL and 1200mg / dL. The KAPPA light chain calibrator was sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C.

[0067] Using the above kit, the samples to be tested were detected and analyz...

Embodiment 2

[0069] The kit for detecting the content of KAPPA light chain comprises: reagent R1, reagent R2 and KAPPA light chain calibrator.

[0070] Wherein, reagent R1 comprises the following components:

[0071]

[0072] Reagent R2 includes the following components:

[0073]

[0074]

[0075] KAPPA Light Chain Calibrators include the following components:

[0076]

[0077] According to the required concentration of the calibrator, add the corresponding amount of KAPPA light chain antigen to the mixture of other reagents above, and mix to obtain the KAPPA light chain calibrator. In this example, 5 different concentrations of KAPPA light chain calibrator were prepared, and the concentrations of the KAPPA light chain antigen were: 0mg / dL, 200mg / dL, 400mg / dL, 800mg / dL and 1200mg / dL. The KAPPA light chain calibrator was sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C.

[0078] Using the above kit, detection and analysis were carried out by Hitach...

Embodiment 3

[0080] The kit for detecting the content of KAPPA light chain comprises: reagent R1, reagent R2 and KAPPA light chain calibrator.

[0081] Wherein, reagent R1 comprises the following components:

[0082]

[0083] Reagent R2 includes the following components:

[0084]

[0085] KAPPA Light Chain Calibrators include the following components:

[0086]

[0087]

[0088] In this embodiment, a high-concentration single-point calibrator is selected, so the concentration of the KAPPA light chain antigen is 1200 mg / dL. The KAPPA light chain calibrator was sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C. When in use, dilute with normal saline to form 5 different concentrations of KAPPA light chain calibrator, the concentrations of the KAPPA light chain antigen are: 0mg / dL, 200mg / dL, 400mg / dL, 800mg / dL and 1200mg / dL.

[0089] Using the above kit, detection and analysis were carried out by Hitachi 7080 automatic biochemical analyzer. The detecti...

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Abstract

The invention discloses a kit for detecting the content of a KAPPA light chain.The kit comprises a reagent R1, a reagent R2 and a KAPPA light chain calibration material, wherein the reagent R1 comprises a first buffer solution, a first electrolyte, a surface active agent, a first stabilizer, a macromolecule accelerant and a first preservative, the reagent R2 comprises a second buffer solution, an anti-human KAPPA light chain antibody, a second electrolyte, a second stabilizer and a second preservative, and the C1 inhibitor calibration material comprises a third buffer solution, a third stabilizer, a third preservative, an antioxidant and a KAPPA light chain antigen.The invention further discloses the application of the kit for detecting the content of the KAPPA light chain and a method for detecting the content of the KAPPA light chain using the kit.By the adoption of the kit and the detection method, the content of the KAPPA light chain can be detected easily and quickly.

Description

technical field [0001] The invention belongs to the technical field of detecting KAPPA light chain content, and in particular relates to a kit, method and application for detecting KAPPA light chain content. Background technique [0002] The light chain (Light chain, L) consists of about 214 amino acid residues, usually does not contain carbohydrates, and has a molecular weight of about 24kD. Each light chain contains two cyclic peptides formed by intrachain disulfide bonds. There are two types of L chains: KAPPA (κ) and Lambda (λ), and the type of L chains on the same natural immunoglobulin molecule is always the same. The κ:λ in normal human serum is about 2:1. [0003] Abnormal proliferation of cell clones will lead to an increase in monoclonal globulin or free immunoglobulin fragments (free light chains), which will cause an imbalance in the ratio of kappa to lambda chains. An abnormal ratio of kappa to lambda predicts monoclonal gamma globulin blood disease. With in...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N21/31G01N35/00
CPCG01N21/31G01N35/00G01N2800/00G01N2800/042G01N2800/085G01N2800/102G01N2800/104
Inventor崔晓辰夏德习王庆国宿明明
Owner潍坊三维生物工程集团有限公司