A method for improving the induction rate of maize haploid induction breeding

Abstract

The invention provides a method for raising the induction rate of corn haploid inducing breeding.The method comprises the steps of conducting cross breeding on a corn haploid inducing line A and a corn haploid inducing line B, so that F1 serving as an inducing line cross-breeding seed C is obtained; taking C as a male parent, and taking a group D used for selecting a new material as a female parent, conducting cross breeding, and harvesting cross breeding current generation grains, so that corn haploid is obtained, wherein cross breeding of C and D comprises the following steps of watering a female parent plant with tap water with the PH value ranging from 5.5 to 8.0; conducting mist spraying treatment on filaments of the female parent plant which is watered with tap water with the same PH value with tap water with the PH value ranging from 5.5 to 8.0; after the filaments of the female parent emerge, conducting pollination cross breeding on the female parent with pollen of the male parent on the fifth day and the seventh day.By means of the method, the haploid inducing rate is effectively raised, breeding efficiency is improved, less investment is input, and the method is simple, convenient and easy to implement; meanwhile, agronomic traits of cross breeding of the inducing lines are greatly improved, and guarantee is provided for efficient and large-scale haploid induction.

Description

technical field [0001] The invention relates to the field of maize haploid induction breeding, in particular to a method for improving the induction rate of maize haploid induction breeding. Background technique [0002] Maize is a monoecious, sexually reproducing crop. The main way to increase maize yield is to use maize heterosis. The focus of maize breeding work is to develop maize hybrids. Breeding maize inbred lines is the basis for cultivating maize hybrids; through crossing between inbred lines to prepare maize combinations and breed new maize hybrids. Therefore, the technology of breeding maize inbred lines is the core technology of maize breeding. The traditional breeding technology of maize inbred lines requires many years of multi-generation self-selection and combining ability measurement technology to breed inbred lines with high combining ability and stable traits; and then breed new maize hybrids through crossbreeding . Breeding a new corn hybrid usually ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for increasing the induction rate of maize haploid induction breeding, which solves the problems of high cost and low induction rate in the existing maize haploid induction breeding technology