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intervention effect of cyanidin-3-o-glucoside on reproductive toxicities of acrylamide and glycidamide
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A technology of glycidyl amide and glucoside, applied in the field of medicine, can solve the problems of ambiguity and less reproductive toxicity research, and achieve the effect of great research significance
Inactive Publication Date: 2016-07-20
GUANGDONG UNIV OF TECH
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However, the toxicological mechanism of AA is still unclear, and there are few reproductive toxicity studies
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Embodiment 1
[0052] The toxicity research of embodiment 1AA / GA to R2C cell
[0053] 1. MTT method to detect the effect of AA / GA on the growth of R2C cells
[0054] (1) Experimental method: use F12 culture medium containing 15% horse serum and 2.5% fetal bovine serum, at 37°C, 5% CO 2 R2C cells were cultured in an incubator, and the medium was changed or passaged every 2 days.
[0055] 1) Adjust the concentration of R2C cells to 4×10 3 cells / mL, inoculate 200 μL of cell suspension in each well of a 96-well plate.
[0056] 2) 24 hours after inoculation, add AA or GA solutions with final concentrations of 0.5, 1, 2, 4, and 6 mmol / L, respectively. A blank group (no cells), a control group (no drug) and a drug group were set up, and 3 replicate wells were set up for each group.
[0057] 3) After stimulating R2C cells with AA or GA for 24 hours, discard the supernatant, add 20 μL of 5 mg / mL MTT, and place in a 37° C. incubator for 4 hours.
[0058] 4) Afterwards, add 200 μL DMSO, place on a...
Embodiment 2C3
[0122] The intervention mechanism of embodiment 2C3G to AA / GA reproductive toxicity
[0123] Through the research of Example 1, the reproductive toxic cell model of AA / GA was successfully established, and the toxic compound IC of AA / GA was selected 50 Group to explore the mechanism of intervention of C3G on the reproductive toxicity of AA / GA.
[0124] 1. MTT assay to detect the effect of C3G on the growth of R2C cells exposed to AA / GA
[0125] (1) R2C cells were cultured to the logarithmic phase, and the cell concentration was adjusted to 4×10 3 cells / mL, inoculate 200 μL of cell suspension in each well of a 96-well plate.
[0126] (2) 24 hours after inoculation, add the final concentration diluted with serum-free culture medium at the same time as IC 50 AA or GA solution and 10, 25, 50, 100 μmol / L C3G solution. Set up blank group (only add culture medium), control group (only add cells), IC 50 AA / GA group and C3G different concentration intervention group (IC 50 AA / GA+C...
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Abstract
The invention discloses application of cyanidin-3-O-glucoside in preparing medicines for preventing and / or treating male genital diseases caused by acrylamide and / or glycidamide. Based upon research results, C3G (the cyanidin-3-O-glucoside) can take an effective intervention effect on injury of R2C cells caused by AA or GA and can inhibit R2C celloxidative stress functional lesion caused by the AA or GA, so that the survival rate of the cells is increased and progesterone synthesis is improved. The C3G can take a relatively good intervention protective effect on R2C cell activity declination, cellapoptosis, progesterone function expression injury and the like exposed by the AA or GA, and the optimum intervention concentration is 10-50[mu]M (M refers to mol / L). Upon research results, the cyanidin-3-O-glucoside provides a novel application;and moreover, the cyanidin-3-O-glucoside provides an important basis for preventing and treating the male genital diseases caused by the AA or GA in human bodies; therefore, the cyanidin-3-O-glucoside has an important research significance.
Description
technical field [0001] The invention belongs to the technical field of medicine. More specifically, it concerns the intervention of cyanidin-3-O-glucoside on the reproductive toxicity of acrylamide and glycidamide. Background technique [0002] Acrylamide (AA), as the main raw material of polyacrylamide, is widely used in drinking water purification, sewage treatment, beauty products, paper industry, mining industry and molecular experiments, which shows that it has a wide range of pollution. People are exposed to AA in multiple ways. In the past, it was considered that the main way of exposure to AA was smoking. However, in the past ten years, it has been found that AA is also contained in a large number of foods. Due to its strong tissue permeability, it can pass through undamaged mucous membranes, The skin, respiratory tract and digestive tract enter the human body, and oral intake is the fastest and most complete way to absorb AA; AA quickly disperses into various tissu...
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