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SiRNA-203 for inhibiting expression of long non-coding RNA SNHG6 and proliferation of hepatoma cells and application thereof

A siRNA-203, liver cancer cell technology, applied in siRNA-203 and application fields, can solve problems such as poor prognosis of liver cancer, and achieve the effects of inhibiting proliferation, great application prospects and economic value

Active Publication Date: 2016-07-20
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, surgery and liver transplantation are still the main treatment methods for liver cancer, interventional chemotherapy, systemic chemotherapy and radiofrequency ablation are also widely used, but the prognosis of liver cancer is still poor
There are no reports about the function of SNHG6

Method used

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  • SiRNA-203 for inhibiting expression of long non-coding RNA SNHG6 and proliferation of hepatoma cells and application thereof
  • SiRNA-203 for inhibiting expression of long non-coding RNA SNHG6 and proliferation of hepatoma cells and application thereof
  • SiRNA-203 for inhibiting expression of long non-coding RNA SNHG6 and proliferation of hepatoma cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Design and obtain siRNA

[0025] Search the SNHG6 gene sequence (ACCESSIONNR_002599.1) in GenBank (http: / / blast.ncbi.nlm.nih.gov / Blast.cgi), and then design siRNA against SNHG6. At the same time, a non-silencing scrambled control (SC) control 19ntsiRNA with no homology to the target gene sequence was designed.

[0026] Select 3 designed siRNAs and designed SC sequences for synthesis, as follows:

[0027] ①siRNA-126:

[0028] The sense strand sequence is: 5'-GAAGGUGUAUGAAAGUCAUTT-3';

[0029] The antisense strand sequence is: 5'-AUGACUUUCAUACACCUUCTT-3';

[0030] ②siRNA-203:

[0031] The sense strand sequence is: 5'-CGGCAUGUAUUGAGCAUAUTT-3';

[0032] The antisense strand sequence is: 5'-AUAUGCUCAAUACAUGCCGTT-3';

[0033] ③siRNA-269:

[0034] The sense strand sequence is: 5'-CUGCUUCGUUACCUCAAGUTT-3';

[0035] The antisense strand sequence is: 5'-ACUUGAGGUAACGAAGCAGTT-3';

[0036] ④SCRNA:

[0037] The sense strand sequence is: 5'-UUCUCCGAACGUGUCACGU-3';

[0...

Embodiment 2

[0065] The results of the example prove that only siRNA-203 has an interference effect on SNHG6. This example shows the biological effect of siRNA-203 down-regulating the expression of SNHG6 in MHCC-LM3 cells. Including changes in cell proliferation and apoptosis.

[0066] (1) Cell proliferation detected by CCK-8 method: Transfect siRNA-203 and SC (3 μg of RNA respectively) into MHCC-LM3 cells according to step (3) of Example 1, and take each group immediately after transfection Cells about 1 x 10 3 Take 3 wells in parallel for each group, put them in the incubator for 1, 2, 3, 4, 5, 6 days, add 10 μl of CCK-8 solution (Dojin Institute of Chemistry, Japan), and incubate at 37°C for 2h, at 450nm The OD value of each group was detected, and the inhibition rate was calculated. The results showed that compared with each control group, the siRNA-203 group could significantly inhibit the proliferation of cells (Table 5, Figure 4 ).

[0067] Table 5 CCK-8 method detects the effe...

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Abstract

The invention discloses siRNA-203 for inhibiting expression of long-chain non-coding RNA SNHG6 and proliferation of hepatoma cells and application thereof. A positive-sense strand of the SiRNA-203 is as shown in SEQ ID NO.1, and an anti-sense strand of the SiRNA-203 is as shown in SEQ ID NO. 2. The siRNA-203 can effectively inhibit the expression of an SNHG6 gene with the down-regulation degree of 10 times, and can effectively inhibit the proliferation of the hepatoma cells and induce the apoptosis of the hepatoma cells. The SiRNA-203 can be further applied to studying functions of the SNHG6 gene and preparing a medicament for treating hepatoma.

Description

technical field [0001] The invention relates to siRNA, in particular to siRNA-203 for inhibiting the expression of long-chain non-coding RNA SNHG6 and the proliferation of liver cancer cells and its application. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma, HCC, hereinafter referred to as liver cancer) is one of the most common malignant tumors in clinical practice. Its global incidence rate has been increasing year by year, exceeding 700,000 per year, ranking the fifth most common malignant tumor incidence rate; The death rate is close to 600,000 per year, ranking third among cancer-related deaths. Liver cancer is highly prevalent in my country, and the number of cases in my country currently accounts for about 50% of the world, ranking second among malignant tumor-related deaths. It can be seen that liver cancer is a serious threat to the life and health of our people and the development of the national economy. Due to the insidious on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/7088A61K48/00A61P35/00
CPCC12N15/1135C12N2310/14C12N2320/12C12N2320/30C12N2320/50
Inventor 刘莉吴德华曹传辉
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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