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Polypeptide with antibacterial activity and application thereof

An antibacterial and antifungal technology, applied in the field of peptides, can solve the problems of complex antibacterial activity prediction of antibacterial peptides and lack of operability, and achieve the effect of clear principle, operability and repeatability

Active Publication Date: 2016-07-27
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the relatively complex antimicrobial activity prediction of antimicrobial peptides and the shortcomings of lack of operability, and provide a method for antibacterial peptide antibacterial activity prediction

Method used

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  • Polypeptide with antibacterial activity and application thereof
  • Polypeptide with antibacterial activity and application thereof
  • Polypeptide with antibacterial activity and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1. Antibacterial peptide antibacterial activity prediction method and principle of the present invention

[0046] In the antibacterial peptide antibacterial activity prediction method of this example, it specifically includes the following steps:

[0047] Step 1. Obtain known antimicrobial peptide sequences and their antibacterial activity data, and use the antibacterial activity standardization conversion to obtain the antibacterial activity standardized value for all antibacterial peptide antibacterial activity data, and randomly select a part of the antibacterial peptide sequence and its antibacterial activity standardized value As a training set, the rest of the antimicrobial peptide sequences and their antibacterial activity normalized values ​​were used as a test set.

[0048] In this step, the method for the standardized conversion of antibacterial activity can be: convert the IC50 value in the unit of mg / L that reflects the activity strength in the an...

Embodiment 2

[0093] Example 2. Establishment of prediction model and design and activity prediction of new antimicrobial peptides

[0094] 1. Obtain known antimicrobial peptide sequences and their antibacterial activity data for the establishment of prediction models. Among all the known sequence data, 80%-90% sequence data are randomly selected as the training set to build the prediction model, and the remaining 10%-20% sequence data are used as the test set to verify the prediction accuracy of the established model. In this modeling, 180 known antimicrobial peptides were used. The amino acid sequence and activity data were all derived from the patent document WO2008 / 022444, which is an antimicrobial peptide with 12 amino acids. 174 antimicrobial peptide sequences were randomly used for each training, and the remaining 14 antimicrobial peptide sequences were used for each test.

[0095] 2. Use antibacterial activity standardization method to standardize antibacterial activity data, and t...

Embodiment 3

[0101] Example three using the method of the present invention to predict and screen antimicrobial peptides

[0102] The present invention starts from the known polypeptide VQLRIRVAVIRA (HH2), uses a computer to randomly change, delete or add mutations at each amino acid residue site, each mutation is a natural amino acid, and obtains a polypeptide sequence of 1,324,256 polypeptides. Choose a peptide sequence library.

[0103] Using the above-mentioned candidate polypeptide sequence library including the template polypeptide to import the two most accurate prediction models established in Example 2 to perform calculations respectively to obtain the activity of each polypeptide, that is, to predict the antibacterial activity of the new polypeptide sequence. The antimicrobial peptides with high predictive activity by the two prediction models were selected as highly active antimicrobial peptides. The prediction models simultaneously calculated their properties such as isoelectri...

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Abstract

The invention belongs to the field of polypeptide and particularly relates to polypeptide with antibacterial activity and application thereof.The purpose is to overcome the defect that the broad-spectrum antibacterial property of existing antibacterial peptide is not good.According to the technical scheme, an antibacterial peptide antibacterial activity prediction method is adopted, polypeptide with broad-spectrum antibacterial activity is obtained, and polypeptide has the amino acid sequence shown in V-Q-W-R-I-R-X1-X2-V-I-R-X3, wherein X1 is equal to I or V, X2 is equal to A or C, X3 is equal to A or R or K, or polypeptide has the amino acid sequence shown in V-Q-L-R-I-R-V-X4-V-I-R-X5, wherein X4 is equal to A or C, and X5 is equal to R or K.It is shown through experiments that a good antibacterial effect is achieved for multiple sensitive bacteria and clinically separated drug-resistant bacteria, low toxicity is achieved for multiple tested normal human body cells, and good application prospects are achieved.

Description

[0001] This application is a divisional application of the invention patent application with the application number 201410241068.9 and the application date is May 30, 2014, and the invention name is "Antibacterial Peptide Antibacterial Activity Prediction Method and Antimicrobial Peptide". technical field [0002] The invention belongs to the field of polypeptides, and in particular relates to polypeptides with antibacterial activity and applications thereof. Background technique [0003] Currently, bacterial infections and diseases are on the rise, and the formation of bacterial resistance is an important reason. The harm caused by drug-resistant bacteria, such as drug-resistant Mycobacterium tuberculosis, Escherichia coli, and methicillin-resistant Staphylococcus aureus (MRSA), known as the "superbug", is becoming more and more serious. The spread of antimicrobial resistance threatens to undo many of the breakthroughs in drug treatment of the last century. Infectious path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04A61P31/10
CPCC07K7/08A61K38/00Y02A50/30
Inventor 杨莉
Owner SICHUAN UNIV
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