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A ratio-type nano-silicon quantum dot fluorescent probe and its preparation method and application
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A nano-silicon quantum dot and silicon quantum dot technology, applied in the fields of nanomaterials and molecular biology, can solve the problems of poor imaging effect and high background, and achieve the effect of stable signal, high sensitivity and high degree of visualization
Active Publication Date: 2018-07-27
FUDAN UNIV
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However, if this probe is directly applied to cell imaging to detect the content of hydroxyl radicals in the body, the strong oxidative properties of hydroxyl radicals can quench the fluorescence, resulting in poor imaging results and high background.
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Embodiment 1
[0036] (1) Preparation of silicon quantum dots
[0037] Before preparation, soak a 25mL round bottom flask with aqua regia (concentrated hydrochloric acid:concentrated nitric acid = 3:1), soak overnight, wash, dry and cool to room temperature. Adjust the speed of the magnetic stirrer, add 1mL triaminopropyl-triethoxysilane (APTES), 5mL water, 1 mL 0.1M sodium ascorbate (NaAA) solution at room temperature, stir for 4h, the solution turns from clear to pink , the silicon quantum dots are produced.
[0038] (2) Synthesis of silicon quantum dots-chlorin e6 composites
[0039] The silicon quantum dots synthesized above (1.9 mL) and 50 μM Ce6 (0.1 mL) were mixed at room temperature and protected from light, and left to stand for two days and two nights, and chlorin e6 was adsorbed to the surface of the silicon quantum dots through electrostatic interaction. , to obtain silicon quantum dots - chlorin e6 composite material, denoted as Si-ce6.
[0040] After obtaining Si-ce6, use a ...
Embodiment 2
[0048] (1) Preparation of silicon quantum dots
[0049] Before preparation, soak a 25mL round bottom flask with aqua regia (concentrated hydrochloric acid:concentrated nitric acid = 3:1), soak overnight, wash, dry and cool to room temperature. Adjust the speed of the magnetic stirrer, add 2 mL of triaminopropyl-triethoxysilane (APTES), 10 mL of water, and 2 mL of 0.1M sodium ascorbate (NaAA) solution at room temperature, stir for 4.5 h, and the solution turns from clear to pink color, silicon quantum dots are produced.
[0050] (2) Synthesis of silicon quantum dots-chlorin e6 composites
[0051] The silicon quantum dots synthesized above (2.5 mL) and 75 μM Ce6 (0.1 mL) were mixed at room temperature and protected from light, and left to stand for two days and two nights, chlorin e6 was adsorbed to the surface of the silicon quantum dots through electrostatic interaction, The silicon quantum dot-chlorin e6 composite material was obtained, denoted as Si-ce6.
[0052] After ob...
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Abstract
The invention belongs to the technical field of nano material science and molecular biology, and particularly relates to a ratio-type nano silicon quantum dot fluorescence probe and a preparation method and application thereof. The preparation method comprises the steps that a one-pot method is adopted, triaminopropyl-triethoxysilane is reduced with sodium ascorbate at room temperature, and water-soluble silicon quantum dots are obtained; the water-soluble silicon quantum dots and chlorin e6 (Ce6) are mixed and subjected to standing at the room temperature under the lucifugal condition, and a silicon quantum dot and chlorin e6 composite material is obtained. The composite material emits fluorescence with the wavelength of 430 nm-580 nm and the wavelength of 640 nm-680 nm under the fluorescence excitation of 410 nm, and active oxygen radicals can specifically quench fluorescence of silicon quanta and basically have no influence on fluorescence of Ce6. Therefore, the composite material can serve as a ratio-type nano silicon quantum dot fluorescence probe to be used for detecting the content of hydroxyl radicals (-OH), rapidness and high efficiency are achieved, the specificity is high, the sensitivity is high, the probe is used for in-vivo cell imaging, the biocompatibility is good, and the visual degree is high.
Description
technical field [0001] The invention belongs to the technical fields of nanomaterials and molecular biology, and specifically relates to a nanometer quantum dot fluorescent probe and a preparation method thereof, as well as an application in high-sensitivity and high-selectivity detection of hydroxyl radicals and cell imaging. Background technique [0002] Silicon quantum dots have good luminescent properties, and have attracted extensive attention from researchers in materials science, chemical biology, and life sciences in recent years. In the past five years, many researchers have done a lot of research on the synthesis method of silicon quantum dots. Silicon quantum dots have the characteristics of good biocompatibility, high fluorescence quantum yield and no photobleaching. They are ideal fluorescent probes in biological imaging applications and have great application value in the field of life sciences. [0003] Reactive oxygen species (Reactive Oxygen Species, ROS) s...
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