PIK3CA gene mutation detection kit

Abstract

The invention provides a PIK3CA gene mutation detection kit, comprising: (1) specific primers for amplifying two exons Exon 9 and 20 of a PIK3CA gene in samples, wherein primer sequences are SEQ ID Nos: 1 to 4 respectively; (2) sequencing primers for sequencing PCR, and conducting sequencing analysis on the two exons Exon 9 and 20 of the PIK3CA gene respectively, wherein primer sequences are SEQ ID Nos: 5 to 6 respectively; (3) two positive quality control tubes with PIK3CA wild type plasmids and mutant type plasmids mixed according to a mass ratio of 1 to 1 and a test tube with PCR reaction premix liquid. The kit has the advantage of detecting the main mutation site type of the PIK3CA gene in the samples through a Sanger sequencing method by directly utilizing paraffin embedding the samples containing some tissue parts of the human body.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for detecting the mutation of a tumor-related gene PIK3CA gene. Background technique [0002] The PIK3CA gene is the intracellular homologue of the retroviral v-p3k oncogene, and the encoded class I phosphatidyl-3-kinases (phosphatidylinositol 3-kinases, PI3Ks) are composed of a p110 catalytic subunit and a p85 regulatory subunit Heterodimer of growth factor receptor tyrosine kinases such as epidermal growth factor receptor (epidermal growth factor receptor, EGFR) and insulin receptor are activated by growth factor receptor tyrosine kinases. After activation, PI3Ks catalyze the phosphorylation of phosphatidylinositol 4-phosphate (PI-4-P) and phosphatidylinositol 4,5-bisphosphate (PI-4,5-P2) into phosphatidylinositol 3,4-bisphosphate (PI -3,4-P2) and phosphatidylinositol 3,4,5-triphosphate (PI-3,4,5-P3, Ptd InsP3). Thereby activating a series of proteins, including regulating cell proliferation, s...

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Herceptin is less effective in people with PIK3CA mutations, study finds

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