Making method of operation vessel for preparation of pig ICSI zygotes

A manufacturing method and a technology of operating dishes, applied in the biological field, can solve the problems of increasing the time for operators to select high-quality sperm, increasing the work intensity of operators, and reducing work efficiency, so as to shorten the time for selecting sperm and reduce the time for selecting sperm , the effect of improving work efficiency

Inactive Publication Date: 2016-08-17
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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Problems solved by technology

In modern technology, when preparing transgenic pigs, a large number of porcine ICSI embryos need to be injected. If the traditional ICSI method is used, the time for the operator to select good sperm is bound to be greatly increased, thereby increasing the work intensity of the operator and reducing the work efficiency.

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  • Making method of operation vessel for preparation of pig ICSI zygotes

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Embodiment Construction

[0022] The implementation of the technical solution will be further described in detail below in conjunction with the accompanying drawings. The examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions. The embodiments described below by referring to the accompanying drawings are exemplary and are intended to explain the present invention, but should not be construed as limiting the present invention. Any modifications, equivalent replacements or Improvements, etc., should be included within the scope of the claims of the present invention, and those not described in detail in this technical solution are all known technologies.

[0023] see figure 1 , a kind of preparation method of pig ICSI zygote of the present invention is used operating dish, and its preparation step and preparation condition are as follows:

[0024] Step 1: Use a...

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Abstract

The invention discloses a making method of an operation vessel for preparation of pig ICSI zygotes. The making method comprises the steps that an operation liquid microdroplet of a set size, a PVP microdroplet for braking, an injection needle treatment microdroplet and a semen culture microdroplet are sequentially made on a phi 60-mm disposable cell culture dish; the semen culture microdroplet comprises a first semen culture microdroplet of an H-shaped structure, a semen screening bridge and a second semen culture microdroplet, and a large quantity of high-quality sperms are selected under the action of the semen screening bridge. The purpose of realizing a large amount of pig ICSI operation in a short time is achieved, time for selecting the sperms in the pig ICSI operation process is shortened, air exposure time of oocytes is shortened, and the survival rate of pig ICSI embryos is increased.

Description

technical field [0001] The invention relates to a method for making an operation dish for preparing porcine ICSI fertilized eggs, and belongs to the field of biotechnology. Background technique [0002] Intracytoplasmic Sperm Injection (Intracytoplasmic Sperm Injection, abbreviation: ICSI, also known as: intracytoplasmic sperm injection technology), research on transgenic pigs and cloned pigs, and donors for xenogeneic organ transplantation With broad application prospects, pigs, as an ideal animal model in the research and application of biotechnology, are increasingly favored by many researchers. However, it is far from enough to rely solely on the collection of embryos in vivo. Therefore, it is very necessary to establish a reasonable in vitro embryo production technology, and a major factor that has long plagued the improvement of the pig in vitro embryo production system is the relatively low incidence of polyspermy. high. As a new technology of livestock embryo engin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0604
Inventor 张立苹郑新民肖红卫刘西梅毕延震李莉华再东华文君任红艳
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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