Rapid identification method of physiological race of Chinese rose powdery mildew bacterium

Abstract

The invention discloses a rapid identification method of physiological race of Chinese rose powdery mildew bacterium. The rapid identification method comprises the following steps: separating, purifying, and propagating Chinese rose powdery mildew bacteria, preparing spore suspension of Chinese rose powdery mildew bacteria, preparing a Chinese rose host material, inoculating, observing the state of powdery mildew, and carrying out statistics, analysis, and identification. In the step of separation, purification, and propagation, powdery mildew bacteria from wild Chinese rose are transferred to leaves of tissue cultured seedlings of rosa odorata; powdery mildew bacteria from cultivated Chinese rose are transferred to leaves of tissue cultured seedlings of purple Chinese rose; 11 domestic identified host materials are chosen, after the step of separation, purification, and propagation, Chinese rose powdery mildew bacteria to be identified are inoculated on 11 domestic identified host materials, and the physiological race can be identified by comparing the physiological race with a comparison table. The method can identify 11 physiological races of Chinese rose powdery mildew bacterium. Only 7 days are needed from inoculation to identification. The method can be used to guide the breeding of Chinese rose species that is resistant to powdery mildew.

Description

technical field [0001] The invention belongs to the technical field of disease-resistant breeding of woody flowers, and in particular relates to a rapid identification method for physiological races of powdery mildew fungus of rose. Background technique [0002] Rose is a plant of the genus Rosa (Rosa L.) in the family Rosaceae. It is currently the most popular garden plant, important potted flower and the largest cut flower in the world. The cut rose (Rosa hybrida) is the largest cut flower in the world, China and Yunnan. [0003] Yunnan Province is the distribution center of my country's wild rose resources and the cultivation center of rose varieties. Since 1994, the output of cut flower roses in Yunnan has ranked first in the country for 15 consecutive years, and its market share in more than 70 large and medium-sized cities in China has exceeded 70%. It is the main production base and distribution center of cut flower roses in my country (Tang Kaixue, Research on the G...

AI Technical Summary

Problems solved by technology

In view of the fact that our country still lacks a set of identification host system suitable for the identification of physiological races of powdery mildew of rose in various regions of the country, the traditional statistical method of host incidence is time-consuming and laborious, and the identification period is long and other technical problems, a method has been explored It is imperative to have a simple, accurate and rapid screening method for the identification of domestic rose powdery mildew physiological races

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