Immune cell culture medium
A technology of immune cells and culture medium, which is applied in the field of cell culture, can solve the problems of slow growth rate and achieve the effects of increased expansion speed, good cell function, and maintenance of cell function
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Embodiment 1
[0017] An immune cell culture medium, including serum-free basal medium, plasma, cytokines and total glycosides of cistanche. The content of total glycosides of Cistanche cistanche was 5 mg / mL, the volume ratio of plasma and serum-free basal medium was 1:15, cytokines included interleukin-2 (10 ng / mL), interleukin-4 (5 ng / mL), interleukin -5 (10 ng / mL), interleukin-12 (15 ng / mL), interleukin-15 (5 ng / mL), interleukin-23 (5 ng / mL), anti-CD3 antibody (100 ng / mL), anti- CD28 antibody (100 ng / mL) and γ-interferon (10 ng / mL).
Embodiment 2
[0019] An immune cell culture medium, including serum-free basal medium, plasma, cytokines and total glycosides of cistanche. The content of total glycosides of Cistanche cistanche was 10 mg / mL, the volume ratio of plasma and serum-free basal medium was 1:20, cytokines included interleukin-2 (15 ng / mL), interleukin-12 (10 ng / mL), interleukin -15 (15 ng / mL), interleukin-23 (15 ng / mL), anti-CD3 antibody (200 ng / mL), anti-CD28 antibody (200 ng / mL) and γ-interferon (10 ng / mL).
Embodiment 3
[0021] An immune cell culture medium, including serum-free basal medium, plasma, cytokines and total glycosides of cistanche. The content of total glycosides of Cistanche cistanche was 15 mg / mL, the volume ratio of plasma and serum-free basal medium was 1:25, cytokines included interleukin-2 (10 ng / mL), interleukin-5 (15 ng / mL), interleukin -12 (15 ng / mL), interleukin-23 (5 ng / mL), anti-CD3 antibody (100 ng / mL), anti-CD28 antibody (100 ng / mL) and gamma-interferon (5 ng / mL).
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