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A kind of hplc fingerprint spectrum of kudzu medicinal material or total flavonoids of kudzu and its establishment method and application

A technology of kudzu and its fingerprints, which is applied to the field of HPLC fingerprinting and establishment of kudzu medicinal materials or total flavonoids of kudzu, achieving good stability and reproducibility

Active Publication Date: 2018-04-27
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of an accurate method to detect the authenticity and quality of the medicinal materials of Pueraria radiata and the quality of the total flavonoids of Pueraria variegata

Method used

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  • A kind of hplc fingerprint spectrum of kudzu medicinal material or total flavonoids of kudzu and its establishment method and application
  • A kind of hplc fingerprint spectrum of kudzu medicinal material or total flavonoids of kudzu and its establishment method and application
  • A kind of hplc fingerprint spectrum of kudzu medicinal material or total flavonoids of kudzu and its establishment method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Establishment of HPLC Fingerprints of Pueraria multibranches or Total Flavonoids of Pueraria multibranches

[0053] 1. Instruments and reagents

[0054] Waters SYNAPT G2-S Q-TOF mass spectrometer (Waters, USA)

[0055] ACQUITY UPLC ultra-high performance liquid chromatography (American waters company)

[0056] ACQUITY UPLC BEH SHIELD RP18 Column (21mm×150mm, 1.7μm)

[0057] AY120 Tray Electronic Analytical Balance (Shimadzu Corporation, Japan)

[0058] DK-S24 Electric Heating Constant Temperature Water Bath (Shanghai Jinghong Experimental Equipment Co., Ltd.)

[0059] LABOROTA4000 rotary evaporator (Heidolph, Germany).

[0060] Acetonitrile is chromatographically pure (Opsen brand), liquid phase water is Watsons distilled water, ethanol is technical ethanol, and other reagents are analytically pure.

[0061] The medicinal materials of Pueraria microphylla were purchased from Yulin, Guangxi, and were identified as Pouzolzia zeylanica (L.) Benn.var.microphy...

Embodiment 2

[0076] Example 2 Identification of characteristic peaks of HPLC fingerprints of kudzu medicinal material or total flavonoids of kudzu

[0077] For the preparation of the test solution, refer to Item 2.1 in Example 1.

[0078] Chromatographic conditions refer to Item 2.2 in Example 1.

[0079] Preparation of reference solution: Accurately weigh 6,7-dimethyl-1,4-dihydro-2,3-quinoxalinedione, apigenin-6-C-a-L-arabinose 8-C-β-D- Glucoside, apigenin-6-C-[α-L-arabinose (1→6)-β-D-glucose], kaempferol-3-O-[α-L-rhamnose (1→6) -β-D-glucose]-7-O-α-L-glucoside, quercetin-3-O-[α-L-rhamnose (1→6)-β-D-glucose]-7- O-α-L-rhamnoside, quercetin-3,7-2-O-α-L-rhamnopyranoside, kaempferol-3-O-[α-L-rhamnose (1→ 6) An appropriate amount of -β-D-glucose]-7-O-α-L-rhamnoside and kaempferol-3,7-2-O-α-L-rhamnopyranoside reference substance is dissolved in an appropriate amount of methanol Configured as a reference solution, that is, too.

[0080] Mass spectrometry detection conditions: electrospray io...

Embodiment 3

[0085] Example 3 The method of using standard fingerprints to detect the authenticity of the medicinal material of Pueraria japonica

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Abstract

The invention relates to the technical field of detection of traditional Chinese medicinal materials, and specifically discloses an HPLC fingerprint spectrum of a medicinal material of Pueraria japonica or total flavonoids of Pueraria japonica and its establishment method and application. The method for establishing the fingerprints includes the following steps: S1. Precisely weighing the medicinal material Pueraria japonica to prepare the test solution; S2. analyzing the test solution by high performance liquid chromatography to obtain the fingerprints. The fingerprints can more comprehensively reflect the characteristic peak information of the sample, and the method has good stability and reproducibility; the fingerprint can be used to verify the true flavonoids of Pueraria multibranches or the total flavonoids of Pueraria multibranches. fake or quality identification.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicinal materials, in particular to an HPLC fingerprint spectrum of a medicinal material or total flavonoids of Pueraria japonica and its establishment method and application. Background technique [0002] Multibranch Mishuige (scientific name: Pouzolzia zeylanica var. microphylla) is a variety of Urticaceae Mishuige. Widely distributed in tropical Asia, produced in southeastern Yunnan, Guangxi, Guangdong, Fujian, southern Jiangxi, and Taiwan; it has detoxification and anti-inflammatory effects; it is used to treat boils and sores. Flavonoids are one of the active components of Pueraria twigs. At present, there is a lack of an accurate method for detecting the authenticity and quality of the medicinal materials of Pueraria radiata and the quality of the total flavonoids of Pueraria variegata. [0003] Traditional Chinese medicine fingerprint refers to the spectrum of c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N30/06
CPCG01N30/06G01N30/8686
Inventor 郭丽冰陶曙红陈卓瀚
Owner GUANGDONG PHARMA UNIV
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