Method for breeding plenty of indoor plutella xylostella with water culture cabbages

A technology of diamondback moth and cabbage, applied in botany equipment and methods, cultivation, application, etc., can solve the problems that nymphs cannot be transferred, are difficult to raise in large quantities, and the cost of cultivation is high, so as to ensure normal development and reproduction, and facilitate large-scale breeding and cultivation The effect of low cost

Inactive Publication Date: 2016-11-16
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used methods for laboratory rearing of diamondback moth are Ke Lidao and Fang Julian (1981) using germinated rapeseed method, Liu Chuanxiu et al. The amount of food provided is limited and it is difficult to raise large quantities. In addition, during the growth and development of diamondback moth larvae, there are many transfer times, some nymphs cannot be transferred, the loss of insects is large, and they are prone to diseases. It is inconvenient to collect and store pupae in large quantities after pupation.
Chen Zongqi et al. (2001) used lotus white as feed for diamondback moth, and Yang Fengshan et al. (2004) raised diamondback moth with cabbage seedlings, and both could obtain a large amount of well-developed insect sources for testing, but the host plant had a long cultivation period and was susceptible to other pests. For example, pests such as cabbage caterpillar and aphids are harmful, and management is difficult. Restricted by climate, soil, site and other conditions, feed cannot be supplied in time, and the cultivation cost is high.
Xu Qin (2005) and Mo Meihua (2007) raised diamondback moths with artificial diets, which required special formulas and were complicated to operate, and general laboratory use had certain limitations.
Lin Kun et al. (2011) used the method of vegetable radish seedlings. Plutella xylostella lay eggs on the vegetable radish seedlings. After the eggs hatched, they were reared with cabbage leaves purchased from the market. They needed to be replaced every 2 days. It was difficult to ensure the freshness of the vegetable leaves. The transfer of Plutella xylostella is unfavorable to the growth of diamondback moth and is susceptible to disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for breeding plenty of indoor plutella xylostella with water culture cabbages
  • Method for breeding plenty of indoor plutella xylostella with water culture cabbages
  • Method for breeding plenty of indoor plutella xylostella with water culture cabbages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1 Materials and methods

[0024] 1.1 Materials and equipment

[0025] Cabbage (Brassica oleracea L.), buy head cabbage (also known as cabbage, cabbage, cabbage, etc.) sold in the market;

[0026] Nutrient solution: Concentrated nutrient solution for home gardening (Shi Kede Gardening Products Company);

[0027] Bug box: 45×45×45cm;

[0028] Culture container: a glass container with a height of 10-15cm and a diameter of 6-10cm;

[0029] Finger tube: 1.5cm in diameter, 6cm in height

[0030] RXZ-380B intelligent artificial climate box (Ningbo Jiangnan Instrument Co., Ltd.);

[0031] NAA (Naphthaleneacetic acid 1-Naphthaleneacetic acid, Shaanxi Senlang Biochemical Co., Ltd.);

[0032] Potassium permanganate (Potassium Permanganate, Zhengzhou Dike Chemical Products Co., Ltd.).

[0033] 1.2 Method

[0034] 1.2.1 Culture of host plants

[0035] Preparation of auxin: Accurately weigh the original drug of NAA, and use 95% alcohol as a solvent to prepare a 1mg / ml mother ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for breeding plenty of plutella xylostella with water culture cabbages. Under the plutella xylostella breeding conditions that the temperature is 24 DEG C, the relative humidity is 70% and the light period L:D is equal to 16:8, water culture is carried out on commercial cabbages, roots are taken well, a single plant of water-culture cabbage can be used for breeding a complete generation of 200 to 300 heads of plutella xylostella, and the biological indicators of the bred plutella xylostella are free of significant difference with those of pot-culture cabbages and vermiculite radish seedling method bred plutella xylostella. The method is simple in operation and low in cost, and fodder is supplied at any time, so that the plenty of indoor plutella xylostella is more convenient to breed.

Description

1. Technical field [0001] The invention relates to a method for raising diamondback moths in large quantities indoors with hydroponic cabbage. 2. Background technology [0002] Diamondback moth Plutella xylostella (L.) belongs to Lepidoptera, Plutellaidae, alias: small caterpillar, two-headed point, hanging silkworm, mainly harms 40 kinds of cruciferous crops such as cabbage, broccoli, mustard greens, cauliflower, Chinese cabbage, rape, radish, etc. , is one of the important pests of cruciferous vegetables worldwide (Feng Xia et al., 2011), and has the ability of long-distance migration (Xing Kun et al., 2015). Plutella xylostella has the characteristics of wide distribution, high fecundity, short life cycle, overlapping generations, and rapid development of drug resistance (Liang Pei et al., 2001), and has become an important research object in the fields of insect drug resistance, physiology, pathology, and toxicology (Yang Fengshan, 2004), is the standard insect species ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/30A01G31/00
CPCA01G31/00A01K67/033
Inventor 尹艳琼谌爱东李向永赵雪晴刘树芳
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products