Tumor ctDNA information statistical method
An information statistics and tumor technology, applied in the biological field, can solve the problems of high cost and complicated experimental operation, and achieve the effect of convenient sample collection, good reproducibility and stability
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[0036] Detection of tumor ctDNA information
[0037] Using the method of the present invention, the ctDNA information of the two groups of liver cancer samples was counted. Among them, the liver cancer samples in the first group are surgical treatment samples, Sample1-1 is the sample before surgical treatment, and Sample1-2 is the sample after surgical treatment; the second group of liver cancer samples is the surgical treatment sample, Sample2-1 is the sample before radiotherapy treatment, Sample2 -2 is the sample after radiotherapy. Such as figure 1 As shown, the specific process is as follows:
[0038] (1) DNA extraction and sequencing: According to the Qiagen plasma DNA extraction manual, extract the free DNA in the plasma of different samples, and directly construct the sample library respectively, in which the two ends of the 100-500bp DNA molecule are added with adapters for sequencing, which come from different samples Different tag sequences are added to the cell-f...
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