Tumor ctDNA information statistical method

An information statistics and tumor technology, applied in the biological field, can solve the problems of high cost and complicated experimental operation, and achieve the effect of convenient sample collection, good reproducibility and stability

Active Publication Date: 2016-11-23
AMOY DIAGNOSTICS CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the changes of tumor-related genes can be detected to a certain extent, there are disadvantages such as high cost, complicated experimental operation, and primer region preference due to target region enrichment operation.

Method used

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  • Tumor ctDNA information statistical method
  • Tumor ctDNA information statistical method

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Experimental program
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Effect test

Embodiment 1

[0036] Detection of tumor ctDNA information

[0037] Using the method of the present invention, the ctDNA information of the two groups of liver cancer samples was counted. Among them, the liver cancer samples in the first group are surgical treatment samples, Sample1-1 is the sample before surgical treatment, and Sample1-2 is the sample after surgical treatment; the second group of liver cancer samples is the surgical treatment sample, Sample2-1 is the sample before radiotherapy treatment, Sample2 -2 is the sample after radiotherapy. Such as figure 1 As shown, the specific process is as follows:

[0038] (1) DNA extraction and sequencing: According to the Qiagen plasma DNA extraction manual, extract the free DNA in the plasma of different samples, and directly construct the sample library respectively, in which the two ends of the 100-500bp DNA molecule are added with adapters for sequencing, which come from different samples Different tag sequences are added to the cell-f...

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Abstract

The invention discloses a tumor ctDNA information statistical method. The tumor ctDNA information statistical method comprises the following steps: (1) DNA extraction and sequencing, (2) sequence comparison, (3) basic statistics, (4) sequencing batch correction, (5) sequence feature correction, (6) copy number variation detection, and (7) fragment difference statistics. The tumor ctDNA information statistical method takes ctDNA as a detection index, and detection can be carried out by only collecting a small amount of peripheral venous blood of a testee. By the tumor ctDNA information statistical method, it is convenient and simple to collect samples, and a detection range can be expanded to terminal patients who are not suitable for collection of samples of biopsy tissues.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a statistical method for tumor ctDNA information. Background technique [0002] At present, tumor has become an important factor affecting human health and quality of life. There are 12.7 million new malignant tumor patients (Male vs Female: 1.1:1) and 7.6 million deaths (1.26:1) every year in the world. According to statistics, 22-30% of the mortality rate is related to smoking, and 10% is related to lifestyle habits such as obesity, alcohol, and inactivity (in developing countries, 22% is related to bacterial infection). In China, the probability of each person suffering from cancer in their lifetime reaches 22% (Male vs Female: 26%: 19%), and the probability of dying of cancer is 13% (17%: 9%). Moreover, tumors can also be called "senile diseases" in a sense, most of them occur after the age of 50 (80%), and the mortality rate is also concentrated after the age of 60...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/24G06F19/22
CPCG16B30/00G16B40/00
Inventor 李旭超黄可君林清华葛会娟施佳卉张福华阮力郑立谋
Owner AMOY DIAGNOSTICS CO LTD
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