A kit for rapid quantitative detection of troponin and creatine kinase isozymes

A troponin, quantitative detection technology, applied in the field of kits, can solve the problems of long detection time and low sensitivity

Active Publication Date: 2017-07-28
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the technical problems of low sensitivity and too long detection time of the troponin and creatine kinase isoenzyme detection kits in the prior art, the purpose of the present invention is to provide a high sensitivity, wide linear range, small cross-interference, and reaction Kit for rapid and quantitatively accurate rapid quantitative detection of troponin and creatine kinase isoenzyme and preparation method thereof

Method used

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  • A kit for rapid quantitative detection of troponin and creatine kinase isozymes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1. A kit for rapid quantitative detection of troponin and creatine kinase isoenzymes

[0063] The kit of Example 1 of the present invention includes a bottom plate (1) on which a sample pad (2), an antibody binding pad I (3), an antibody binding pad II (4), and a coating layer Two detection lines and one quality control line are coated with an analysis membrane (5) and an absorbent pad (6), wherein the antibody binding pad I is coated with a biotin-labeled anti-troponin I monoclonal antibody coupling The antibody binding pad II is coated with immunomagnetic bead II coupled with an anti-creatine kinase isoenzyme monoclonal antibody, and the surface of the magnetic bead I is coated with streptavidin, The two detection lines are detection line I (501) coated with troponin I monoclonal antibody and detection line II (501) coated with creatine kinase isoenzyme monoclonal antibody. The quality control line (502) ) Anti-mouse IgG antibody is immobilized.

[0064] Preparat...

Embodiment 2

[0087] Example 2. A kit for rapid quantitative detection of troponin and creatine kinase isoenzymes

[0088] The kit described in Example 2 of the present invention includes a bottom plate on which a sample pad, an antibody binding pad I, and an antibody binding pad II are sequentially overlapped, and are coated with two detection lines and a quality control line. Analysis membrane and absorbent pad, wherein the antibody binding pad I is coated with immunomagnetic beads I coupled with a biotin-labeled anti-troponin I monoclonal antibody, and the antibody binding pad II is coated with an anti-muscle Acid kinase isoenzyme monoclonal antibody coupled immunomagnetic bead II, the surface of the magnetic bead I is coated with streptavidin, the two detection lines are for the detection of the troponin I monoclonal antibody Line I and detection line II coated with creatine kinase isoenzyme monoclonal antibody, the quality control line is fixed with anti-mouse IgG antibody.

[0089] Repeat...

Embodiment 3

[0090] Example 3. A kit for rapid quantitative detection of troponin and creatine kinase isoenzymes

[0091] The kit described in Example 3 of the present invention includes a bottom plate, and the bottom plate is provided with a sample pad, an antibody binding pad I, and an antibody binding pad II that are sequentially overlapped with two detection lines and a quality control line. Analysis membrane and absorbent pad, wherein the antibody binding pad I is coated with immunomagnetic beads I coupled with a biotin-labeled anti-troponin I monoclonal antibody, and the antibody binding pad II is coated with an anti-muscle Acid kinase isoenzyme monoclonal antibody coupled immunomagnetic bead II, the surface of the magnetic bead I is coated with streptavidin, the two detection lines are for the detection of the troponin I monoclonal antibody Line I and detection line II coated with creatine kinase isoenzyme monoclonal antibody, the quality control line is fixed with anti-mouse IgG antib...

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Abstract

The invention belongs to the field of kits, and particularly relates to a kit for rapidly and quantitatively detecting troponin and creatine kinase isozyme, and a preparation method of the kit. The kit comprises a base plate, wherein a sample pad, an antibody binding pad I, an antibody binding pad II, an enveloped analysis film and a water absorption pad which are sequentially lapped are arranged on the base plate; an immunomagnetic bead which is coupled with biontin-marked anti-troponin I monoclonal antibody is enveloped on the antibody binding pad I, an immunomagnetic bead which is coupled with an anti-creatine kinase isozyme monoclonal antibody is enveloped on the antibody binding pad II, and the surface of the magnetic bead I is enveloped with streptavidin. The kit has the advantages of being simple and convenient to operate, fast to react, high in sensitivity, strong in specificity, suitable for on-site detection and the like, and is suitable for large-range popularization and application.

Description

Technical field [0001] The invention belongs to the field of kits, and specifically relates to a kit for rapid quantitative detection of troponin and creatine kinase isoenzymes. Background technique [0002] Cardiovascular disease poses a great threat to human health and life. It has become one of the two major medical difficulties. Cardiovascular disease is the "top killer" leading to human deaths in the world. There are nearly 200 million cardiovascular disease patients in my country every year. Nearly 3 million people died of cardiovascular disease. Therefore, it is extremely urgent to establish a detection system that can accurately, quickly and conveniently predict and diagnose cancer and cardiovascular diseases. [0003] Cardiovascular markers mainly include troponin I, troponin T, creatine kinase (CK) and creatine kinase isoenzyme (CK-MB), myoglobin, D-dimer, C-reactive protein, etc. [0004] Cardiac Troponin I (Cardiac Troponin I, referred to as cTnI) is a specific and sensi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/573G01N33/543
CPCG01N33/54326G01N33/573G01N33/68G01N2800/32
Inventor 陈立国
Owner ANHUI IPROCOM BIOTECH CO LTD
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