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A kind of amino acid sequence and application of detecting plasma pou5f1 natural antibody

A POU5F1, natural antibody technology, applied in the detection of the amino acid sequence of the plasma POU5F1 natural antibody and its application field, can solve the problem of high risk of tumors and achieve the effect of predicting the risk of tumor occurrence

Inactive Publication Date: 2019-09-06
长春市海兰深生物医学技术有限公司
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Problems solved by technology

Based on this, it is speculated that the risk of developing tumors in people with natural antibody deficiency may be significantly higher than that in normal people

Method used

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  • A kind of amino acid sequence and application of detecting plasma pou5f1 natural antibody
  • A kind of amino acid sequence and application of detecting plasma pou5f1 natural antibody
  • A kind of amino acid sequence and application of detecting plasma pou5f1 natural antibody

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Embodiment Construction

[0023] 1. Sample collection: 132 plasma samples from patients with hepatocellular carcinoma (Hepatocellular Carcinoma, HCC) (including 111 males and 21 females, with an average age of 54.9±9.9 years) and 231 plasma samples from healthy people (including 184 males and Forty-seven females, with an average age of 55.2±10.9 years) were used to detect the level of natural antibody to POU5F1 in plasma. All plasma samples were stored at minus 80 degrees (-80°C) before operation, and it was verified that the storage time did not exceed two years, and there were no plasma samples that had been repeatedly frozen and thawed (freezing and thawing times did not exceed 3 times).

[0024] 2. Sample detection: Thaw plasma samples at 4°C. The two peptide polyantigens used in this experiment were synthesized by SEVERN BIOTECH Co., Ltd., UK, with a purity of 95%. The specific operations are as follows:

[0025] (1) Before operation, each antigen was dissolved in 67% acetic acid into a 5 mg / ml st...

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Abstract

The invention discloses an amino acid sequence used for detecting blood plasma POU5F1 natural antibodies, and applications thereof. The amino acid sequence is H-HFTALYSSVPFPEGEAFPPVSVTTLGSPMHSN-OH H-ALQKELEQFAKLLKQKRITLGYTQADVGLTLH-OH, the purity is higher than 95%, and pH value is higher than 7.0. The invention also discloses applications of the immunological marker POU5F1 natural antibodies in rumor risk prediction and clinical tumor prevention. Benefit effects are that: the amino acid sequence can be used for qualitative and quantitative determination of the corresponding natural antibodies, tumor risk prediction is promising to realize, and reliable data is provided for tumor novel drug research and development.

Description

technical field [0001] The present invention relates to an amino acid sequence and application of a natural antibody, in particular to an amino acid sequence and application of a natural antibody for detecting plasma POU5F1. Background technique [0002] At present, relevant studies have shown that 3-5 years before the development of malignant tumor volume can be detected by modern imaging techniques, high concentrations of tumor-associated antigen autoantibodies can appear in the blood of patients. Therefore, the detection of tumor-associated antigen autoantibodies in blood has important value in predicting the risk of tumor incidence and early diagnosis of tumors. Early diagnosis kits for diagnosing lung cancer and breast cancer are commercially available abroad. For example, Early CDT launched by Oncimmune Ltd., Nottingham, UK TM -Lung diagnostic kit has been used in clinical early diagnosis of lung cancer in North America and Europe for nearly 6 years. However, the cu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00G01N33/574G01N33/68
Inventor 尉军张萱
Owner 长春市海兰深生物医学技术有限公司
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