Rapid detection method for psoralen and isopsoralen
A technology of isopsoralen and psoralen, which is applied in the field of rapid detection of psoralen and isopsoralen content, can solve the problems of complex sample processing process and long overall analysis time, and save analysis time , Improve analytical sensitivity, rapid and accurate detection effect
Active Publication Date: 2015-12-09
大连润生康泰医学检验实验室有限公司
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The sample processing process of this analytical method is complex and the overall analysis time is long
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[0024] A method for detecting psoralen and isopsoralen based on PY-GC / MS, taking psoralen preparation as an example, but the protection scope of the present invention is not limited to this embodiment, and the specific steps are as follows:
[0025] 1. Sample preparation
[0026] Accurately weigh 1.00 mg (±0.02 mg) of the obtained Bakuchia Capsules (Shaanxi Snow Biotechnology Co., Ltd., Xi'an) into a 50 μL pyrolysis cuvette;
[0027] 2. Pyrolysis:
[0028] The pyrolysis furnace adopts Frontiers PY2020is;
[0029] ①Pyrolysis temperature: 300℃;
[0030] ②Pyrolysis time: 0.20min.
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The invention provides a rapid detection method for psoralen and isopsoralen. The method comprises steps as follows: 1) preparation of samples; 2) PY-GC / MS (pyrolysis-gas chromatography / mass spectrum) detection; 3) data analysis as well as qualitative and quantitative analysis. A PY-GC / MS technology is selected for detecting the content of the psoralen and the isopsoralen, according to the problem of different quality due to differences in origins, seasons, preparation methods and the like of Chinese angelica in actual production and life, the rapid and trace detection method for the psoralen and the isopsoralen is provided, and then the quality of fructus psoraleae is preliminarily judged; the method has the characteristics of simplicity in operation, low detection limit, good experimental reproducibility, direct and reliable experimental data and the like, is particularly applicable to trace sample detection, can be used for preliminarily comparing quality differences of different varieties and different batches of fructus psoraleae samples from different origins, is wide in application range and provides a preliminary judgment basis for the quality standard and the modernization application of the fructus psoraleae.
Description
technical field [0001] The invention relates to a method for rapidly detecting the content of psoralen and isopsoralen based on fast pyrolysis-gas chromatography-mass spectrometry (PY-GC / MS method). Background technique [0002] Psoraleae is the dried and mature fruit of the leguminous plant Psoraleae, which has the effects of warming the kidney and supporting yang, inducing Qi and stopping diarrhea. Its main components are coumarins and flavonoids, among which psoralen and isopsoralen are important effective components. As traditional Chinese medicine, psoralen and isopsoralen are often used to prevent and treat osteoporosis. Modern pharmacological experiments show that psoralen and isopsoralen are effective Ca + Channel antagonist, which can significantly promote the proliferation and differentiation of rat osteoblasts. [0003] Recent studies have shown that psoralen and isopsoralen can be used in the treatment of T-cell lymphoma, human breast cancer cells and some auto...
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IPC IPC(8): G01N30/02
Inventor 陈福欣龚频周安宁张少利
Owner 大连润生康泰医学检验实验室有限公司