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Kit for screening colorectal cancer hereditary susceptibility genes

A susceptibility gene, colorectal cancer technology, applied in the field of molecular biology and medicine, can solve the problems of high cost and low detection efficiency, achieve the effect of reducing detection cost, high accuracy and sensitivity, and improving detection efficiency

Inactive Publication Date: 2016-12-21
SICHUAN KINGMED DIAGNOSTICS CENT
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

At present, the common method for detecting colorectal cancer susceptibility genes is Sanger sequencing method, which can only sequence a certain region of a sample, and the detection efficiency is low and the cost is high

Method used

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Examples

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Embodiment 1

[0016] A colorectal cancer hereditary susceptibility gene screening kit, the operation method comprises the following steps,

[0017] 1) Use specific primers for colorectal cancer susceptibility genes to amplify multiple target regions in the sample to be tested, and construct a sequencing library based on the amplified products;

[0018] 2) Perform single-molecule amplification on the sequencing library to obtain single-molecule amplification products corresponding to multiple target regions;

[0019] 3) Simultaneously performing high-throughput gene sequencing on multiple single-molecule amplification products to obtain sequence information of the multiple target regions.

[0020] Among them, the target region is any sequence of colorectal cancer susceptibility gene, which can be selected according to needs, including the internal sequence of the gene and the external regulatory region of the gene, wherein the internal sequence of the gene includes the intron region of the c...

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PUM

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Abstract

The invention discloses a kit for screening colorectal cancer hereditary susceptibility genes. The kit comprises colorectal cancer susceptibility gene specificity primers for amplifying a plurality of target regions in a to-be-detected sample, wherein the colorectal cancer susceptibility genes include at least one of rs10795668, MMP2, SMAD7, ADH2, ALDH2, CYP1A2, MMP-1, MTHFR, TP53, VEGF, COX-2, DNMT3B, hMLH1, LOC727677, MMP9, MTRR and TGF-beta 1. The kit can detect a plurality of regions of the colorectal cancer susceptibility genes at the same time, detecting efficiency is improved, detecting cost is reduced, and detecting accuracy and sensitivity are high.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, in particular to a screening kit for colorectal cancer hereditary susceptibility genes. Background technique [0002] Colorectal cancer (CRC), also known as colorectal cancer, is a general term for colon cancer and rectal cancer. One, its mortality rate is high. Globally, colorectal cancer is one of the most common malignant tumors. Its morbidity and mortality are second only to gastric cancer among malignant tumors of the digestive tract and rank fourth among all malignant tumors. Colorectal cancer is easy to be misdiagnosed. When the stool frequency increases in the early stage, mucus and pus and blood appear in the stool, it is easy to be misdiagnosed as dysentery, enteritis or hemorrhoids and other diseases, thus losing the opportunity for early treatment. [0003] Colorectal cancer susceptibility genes are genes that may be associated with colorectal cancer in preliminary stu...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/16
Inventor 黄平欧阳小峰毛家丽陈德忠
Owner SICHUAN KINGMED DIAGNOSTICS CENT
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